Effects of curcumin combined with cisplatin on expressions of Bcl-2 and Caspase-3 in lung cancer A549 cells

FU Yazhai; YANG Linjun; JIN Yuanyuan

您当前的位置：

首页 >

文章列表页 >

Effects of curcumin combined with cisplatin on expressions of Bcl-2 and Caspase-3 in lung cancer A549 cells

更新时间：2022-08-31

- Effects of curcumin combined with cisplatin on expressions of Bcl-2 and Caspase-3 in lung cancer A549 cells
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 1, Pages: 95-98(2018)
- 作者机构：
  
  1. 浙江省台州市博爱医院肿瘤内科,浙江,台州,318050
  2. 上海长海医院肿瘤科,上海,200433
  3. 浙江省台州市路桥君同肿瘤医院,浙江,台州,318050
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
FU Yazhai, YANG Linjun, JIN Yuanyuan. Effects of curcumin combined with cisplatin on expressions of Bcl-2 and Caspase-3 in lung cancer A549 cells. [J]. Shanghai Journal of Traditional Chinese Medicine 52(1):95-98(2018)
DOI：

FU Yazhai, YANG Linjun, JIN Yuanyuan. Effects of curcumin combined with cisplatin on expressions of Bcl-2 and Caspase-3 in lung cancer A549 cells. [J]. Shanghai Journal of Traditional Chinese Medicine 52(1):95-98(2018) DOI：

摘要

目的：探讨姜黄素联合顺铂对肺癌A549细胞Bcl-2及caspase-3表达的影响。方法：选用肺癌A549细胞分为对照组、姜黄素组、顺铂组、联合用药组,分别采用相应药物进行处理。采用MTT法检测细胞增殖抑制率,流式细胞术检测细胞凋亡率和细胞周期,RT-PCR法检测细胞Bcl-2 mRNA、caspase-3 mRNA表达水平,Western blot法检测细胞Bcl-2、caspase-3蛋白表达水平。结果：①与对照组比较,其余各组24 h、48 h、72 h肺癌A549细胞增殖抑制率较高,且联合用药组高于姜黄素组、顺铂组,差异有统计学意义(P<0.05)。②与对照组比较,其余各组肺癌A549细胞凋亡率较高,且联合用药组高于姜黄素组、顺铂组(P<0.05)；姜黄素组存在G2/M期细胞阻滞,顺铂组及联合用药组存在S期细胞阻滞。③与对照组比较,其余各组肺癌A549细胞Bcl-2 mRNA及Bcl-2蛋白表达水平较低,其中联合用药组＜顺铂组＜姜黄素组(P<0.05)。④与对照组比较,其余各组肺癌A549细胞caspase-3 mRNA及caspase-3蛋白表达水平较高,其中联合用药组＞顺铂组＞姜黄素组(P<0.05)。结论：姜黄素联合顺铂能够抑制肺癌A549细胞Bcl-2表达、促进caspase-3表达,以抑制肺癌A549细胞增殖并促进其凋亡。

Abstract

Objective:To discuss the effects of curcumin combined with cisplatin on the expressions of Bcl-2 and caspase-3 in the lung cancer A549 cells. MethodsThe lung cancer A549 cells were divided into the control group, curcumin group, cisplatin group and combination group. Each group was treated with the corresponding drugs. The inhibition rate of cell proliferation was detected by MTT assay. The cell apoptosis rate and cell cycle were detected by flow cytometry. The expression levels of Bcl-2 mRNA and caspase-3 mRNA were detected by RT-PCR,and the protein expression levels of Bcl-2 and caspase-3 were detected by Western blot. Results:①Compared with the control group,the inhibition rates of cell proliferation were increased in the other groups after 24-,48- and 72-hour drug treatment,and the inhibition rate of the combination group was higher than that of the curcumin group or cisplatin group,with statistically significant differences(P<0.05). ②Compared with the control group,the cell apoptosis rates were increased in the other groups,and the apoptosis rate of the combination group was higher than that of the curcumin group or cisplatin group(P<0.05). The cell arrest in G2/M phase was observed in the curcumin group,and the cell arrest in S phase was found in the cisplatin group and combination group. ③Compared with the control group,the mRNA and protein expression levels of Bcl-2 in lung cancer A549 cells were decreased in the other groups. The expression levels in the combination group were lower than those in the cisplatin group,and the expression levels in the cisplatin group were lower than those in the curcumin group(P<0.05). ④Compared with the control group,the mRNA and protein expression levels of caspase-3 in lung cancer A549 cells were increased in the other groups. The expression levels in the combination group were higher than those in the cisplatin group,and the expression levels in the cisplatin group were higher than those in the curcumin group(P<0.05). Conclusion:Curcumin combined with cisplatin can inhibit the expression of Bcl-2 and promote the expression of caspase-3 in lung cancer A549 cells,and thus inhibit the cell proliferation and promote the cell apoptosis.

关键词

姜黄素肺癌A549细胞Bcl-2caspase-3

Keywords

curcuminlung cancerA549 cellsBcl-2caspase-3

references

Views

302

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Yushen Zhuyun decoction on expression of Bcl-2, Bax and Caspase-3 in ovaries of rats with diminished ovarian reserve

Distribution rule of TCM syndrome elements in chronic obstructive pulmonary disease with lung cancer

Discussion on traditional Chinese medicine pathogenesis of brain metastases from lung cancer based on theory of “lung⁃brain correlation”

Discussion on inflammatory⁃cancer transformation of pulmonary nodules and traditional Chinese medicine prevention and treatment strategies based on theory of latent toxicity due to vital qi deficiency

Effect and mechanism of action of Qingfei Tongluo Formula on oxidative stress injury induced by Mycoplasma pneumoniae in A549 cells

Related Author

No data

Related Institution

Department of Gynecology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine

Department of Traditional Chinese Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine

Suzhou Hospital of Traditional Chinese Medicine

Shanghai Pulmonary Hospital， Tongji University

Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

⁰