Objective:To optimize the best compatibility of berberine, emodin and cinnamaldehyde in anti-hepatoma activity by uniform design. MethodsThe nude mice were subcutaneously injected with human hepatoma cells under the right axillary to establish the transplantation tumor model. Based on uniform design table U,6,(6,4,),the grouping design of berberine, emodin and cinnamaldehyde was performed according to “3 factors and 6 levels”. And each group was treated with the corresponding ingredients compatibility. During two-week administration,the changes on the state,death,body weight and tumor volume of mice were observed. After the mice were sacrificed,the tumor was weighed and the tumor inhibition rate was calculated. And the pathological sections of tumor were regularly made. Taken the tumor inhibition rate as primary indicator,the best compatibility of these three ingredients was obtained by regression analysis of uniform design.  Results:①No abnormal state and death were found in mice of all groups. ②There was a decreasing trend on body weight of mice in each drug treatment group. ③Compared with the model group,the decreasing trend on tumor volume and tumor weight were observed in each drug treatment group,which suggested that all the treatments showed the tumor inhibitory effects with different degrees. Among,the tumor inhibition rate was 23% in the group P4,and its effect was more significant. ④The characteristic morphology of tumor was observed in the pathological sections,including cellular size and shape,nucleoplasm ratio,karyotype and intercellular matrix. Among,the morphological changes in the group P4 were better than those in the other groups. ⑤After screening,the regression equation was Y=1.95+1.302A-0.253B+0.290C. It meant,when berberine (A),cinnamaldehyde (C) and emodin (B) were 10 μg/g,50 μg/g and 25 μg/g respectively,the compatibility of these three ingredients showed the best inhibitory effects on tumor.  Conclusion:The compatibility of berberine, emodin and cinnamaldehyde can effectively inhibit the growth of hepatocellular carcinoma tissue, and the best compatibility ratio is 2∶5∶10.