Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency

CHEN Bin; SUN Jue; LIANG Fang; XU Ke; WAN Guangsheng; YUAN Xu

您当前的位置：

首页 >

文章列表页 >

Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency

更新时间：2022-08-31

- Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency
- Shanghai Journal of Traditional Chinese Medicine Vol. 52, Issue 1, Pages: 81-85(2018)
- 作者机构：
  
  1. 上海中医药大学附属普陀医院中医肿瘤科,上海,200062
  2. 上海中医药大学中西医结合肿瘤介入研究所,上海,200062
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
CHEN Bin, SUN Jue, LIANG Fang, et al. Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency. [J]. Shanghai Journal of Traditional Chinese Medicine 52(1):81-85(2018)
DOI：

CHEN Bin, SUN Jue, LIANG Fang, et al. Regulation of Jianpi Jiedu Recipe on intestinal flora in tumor bearing mice with syndrome of spleen deficiency. [J]. Shanghai Journal of Traditional Chinese Medicine 52(1):81-85(2018) DOI：

摘要

目的：探讨健脾解毒方对脾虚荷瘤鼠移植瘤的抑制作用、对脾虚小鼠肠道菌群的调节作用及两者之间的相关性。方法：48只小鼠随机取36只,利用番泻叶水煎剂成功制备脾虚模型,剩余12只为N.S.组,再使用CT26细胞构建移植瘤模型。36只脾虚模型小鼠则随机分为脾虚组、健脾解毒方低剂量组、健脾解毒方高剂量组,每组12只。连续灌胃给药4周后脱颈椎处死各组小鼠,称取瘤重,计算抑瘤率；计算胸腺指数和脾指数；流式细胞仪检测CD4,+,、CD8,+, T细胞比例,ELISA法检测血清中IL-17水平；16S rRNA基因测序法检测肠道菌群多样性。结果：脾虚组平均瘤重、血清IL-17水平高于N.S.组和健脾解毒方低、高剂量组,同时脾虚组胸腺指数、脾指数、外周血CD4,+,T细胞比例、CD4,+,/CD8,+,比值低于N.S.组和健脾解毒方低、高剂量组,差异均有统计学意义(P,<,0.01)；且脾虚组小鼠肠道菌群中拟杆菌门显著增多、厚壁菌门显著减少,存在明显的菌群失调现象。与N.S.组及健脾解毒方低、高剂量组比较,脾虚组小鼠肠道中双歧杆菌及乳杆菌减少,而肠杆菌及肠球菌增多。结论：健脾解毒方具有抑制脾虚荷瘤鼠皮下瘤生长、提高CD4,+,/CD8,+,比例、减少炎症因子IL-17分泌的作用,这些作用可能与其改善脾虚小鼠的肠道菌群失调相关。

Abstract

Objective:To explore the inhibitory effects of Jianpi Jiedu Recipe on the transplanted tumor in tumor bearing mice with syndrome of spleen deficiency and the regulatory effects on the intestinal flora in mice with syndrome of spleen deficiency,and discuss the relationship between the two. Methods36 mice from total 48 mice were randomly taken and the mice model of spleen deficiency was established by water decoction of senna. The remaining 12 mice were taken as the normal saline(N.S.)group. Then the model of transplanted tumor was established by CT 26 cells. The 36 model mice of spleen deficiency were randomly divided into the spleen deficiency group and Jianpi Jiedu Recipe(JPJD)groups with low-and high-dose,12 mice in each group. After intragastric administration for continuous 4 weeks,the mice in each group were sacrificed. The tumor was weighed and the inhibition rate was calculated. The thymus index and spleen index were calculated. The proportion of CD4,+, and CD8,+,Tcells was detected by flow cytometry. The serum level of IL-17 was detected by ELISA. The diversity of intestinal flora was determined by 16S rRNA gene sequencing. Results:The average tumor weight and the serum level of IL-17 in the spleen deficiency group were higher than those in the N.S. group and JPJD groups with low-and high-dose,and the thymus index and spleen index,the proportion of CD4,+, T cells in peripheral blood and CD4,+,/CD8,+, ratio in the spleen deficiency group were lower than those in the N.S. group and JPJD groups with low-and high-dose,with statistically significant differences(P,<,0.01). Also,bacteroides was significantly increased and firmicutes was significantly decreased in the intestinal flora of mice in the spleen deficiency group,which showed the obvious dysbacteria. Compared with the N.S. group and JPJD groups with low-and high-dose,bifidobacteria and lactobacillus were decreased and enterobacteria and enterococcus were increased in the spleen deficiency group. Conclusion:Jianpi Jiedu Recipe can inhibit the growth of subcutaneous tumor in tumor bearing mice with syndrome of spleen deficiency,increase the ratio of CD4,+,/CD8,+, and decrease the secretion of IL-17. These effects may be associated with the improvement on alteration of intestinal flora in mice with spleen deficiency.

关键词

健脾解毒方脾虚大肠癌小鼠模型肠道菌群失调CD4+/CD8IL-17

Keywords

JianPi JieDu Recipespleen deficiencycolorectal cancermouse modelalteration of intestinal floraCD4+/CD8IL-17

references

Views

439

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4⁺ CD25⁺ Foxp3T/Th17 cells

Effects of Yiqi Fusheng Recipe combined with chemotherapy on tumor growth and tumor infiltrating lymphocytes in tumor-bearing mice with gastric cancer

Mechanism of Jianpi Jiedu Formula to inhibit epithelial mesenchymal transition of colorectal cancer through Hippo signaling pathway

Effects of medicated serum of Compound Shenlu Granule on TNF-α induced CD34⁺ cell apoptosis in normal bone marrow based on P38MAPK pathway

Effect of Jianpi Jiedu Recipe on colorectal cancer cells via COX-2/β-catenin/MMP-7 signal pathway

Related Author

No data

Related Institution

Jiangsu Provincial Hospital of TCM

Nanjing Chinese Medical University

Department of Oncology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

Frist Department of Oncology, The TCM Hospital Affiliated to Xinjiang Medical University

Department of Medical Oncology and Cancer Institute， Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

⁰