Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice

WANG Song; XI Qing; LI Guowen; CHANG Jun; YU Peizhong; SHEN Chaobin

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Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice

更新时间：2022-08-31

- Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice
- Shanghai Journal of Traditional Chinese Medicine Vol. 51, Issue 12, Pages: 67-70(2017)
- 作者机构：
  
  1. 上海中医药大学附属上海市中西医结合医院,上海,200082
  2. 复旦大学,上海,201203
  3. 上海市中医老年医学研究所,上海,200031
- 作者简介：
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WANG Song, XI Qing, LI Guowen, et al. Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice. [J]. Shanghai Journal of Traditional Chinese Medicine 51(12):67-70(2017)
DOI：

WANG Song, XI Qing, LI Guowen, et al. Effects of astragaloside IV on Th cell subsets in OVA-induced asthmatic mice. [J]. Shanghai Journal of Traditional Chinese Medicine 51(12):67-70(2017) DOI：

摘要

目的：观察黄芪甲苷(AST IV)对卵白蛋白(OVA)诱导的哮喘小鼠脾源性Th1及Th2的免疫调节作用。方法：建立OVA诱导的小鼠哮喘模型,分离培养脾单个核细胞,加入不同浓度的黄芪甲苷。采用酶联免疫吸附法(ELISA)测定细胞培养液上清液细胞因子IFN-γ、IL-4及 IL-13的表达；流式细胞术(FCM)在单个核细胞内测定IFN-γ、IL-4及IL-13的百分含量。结果：与OVA组比较,不同剂量黄芪甲苷(25 mg/L、50 mg/L、100 mg/L)对OVA诱导的哮喘小鼠Th细胞亚群分泌的细胞因子IFN-γ、IL-4及IL-13均有一定的抑制作用(P<0.05)。结论：黄芪甲苷对OVA诱导的Th2细胞应答有抑制作用,通过抑制Th1及Th2细胞达到免疫调节作用。

Abstract

Objective:To observe the immune regulatory effects of astragaloside IV on spleen derived Th1 and Th2 cells in ovalbumin(OVA)induced asthmatic mice. MethodsThe OVA-induced mouse asthma model was established. The spleen mononuclear cells were isolated and cultured and intervened by astragaloside IV at different concentrations. The expression levels of cytokines including IFN-γ,IL-4 and IL-13 in the supernatant of cell culture medium were detected by enzyme linked immune sorbent assay (ELISA),and the percentage of IFN-γ,IL-4 and IL-13 within mononuclear cells were detected by flow cytomentry. Results:Astragaloside IV at the concentrations of 25,50 and 100 mg/L showed certain inhibitory effects on the cytokines(IFN-γ,IL-4 and IL-13)secreted by Th cell subsets in OVA-induced asthmatic mice(P<0.05). Conclusion:Astragaloside IV shows the inhibitory effects on OVA-induced Th2 cell response,and its immune regulation effects may be associated with the inhibition on Th1 and Th2 cells.

关键词

黄芪甲苷免疫调节体外实验Th细胞亚群

Keywords

Astragaloside IVimmune regulationexperiment in vitroTh cell subsets

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