Experimental study on intervention of baicalin and astragaloside IV on Pseudomonas aeruginosa biofilm in vitro

LI Shao-bin; FANG Hong; CHENG Yan-qi; GONG Shao-kang; WANG Pei-yuan; WU Yin-gen

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Experimental study on intervention of baicalin and astragaloside IV on Pseudomonas aeruginosa biofilm in vitro

更新时间：2022-08-31

- Experimental study on intervention of baicalin and astragaloside IV on Pseudomonas aeruginosa biofilm in vitro
- Shanghai Journal of Traditional Chinese Medicine Vol. 51, Issue 11, Pages: 63-67(2017)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院中医预防保健科,上海,200032
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LI Shao-bin, FANG Hong, CHENG Yan-qi, et al. Experimental study on intervention of baicalin and astragaloside IV on Pseudomonas aeruginosa biofilm in vitro. [J]. Shanghai Journal of Traditional Chinese Medicine 51(11):63-67(2017)
DOI：

LI Shao-bin, FANG Hong, CHENG Yan-qi, et al. Experimental study on intervention of baicalin and astragaloside IV on Pseudomonas aeruginosa biofilm in vitro. [J]. Shanghai Journal of Traditional Chinese Medicine 51(11):63-67(2017) DOI：

摘要

目的：研究黄芩苷、黄芪甲苷对铜绿假单孢菌生物膜形成的影响。方法：取铜绿假单胞菌PAO1标准株,用黄芩苷、黄芪甲苷对PAO1生物膜的体外培养进行干预,以阿奇霉素作为实验体系阳性对照,0.9%NaCl溶液为生理盐水阴性对照组。FITC-ConA染色,于共聚焦显微镜下观察不同培养条件下的生物膜的形态；同时,TRIzol法提取总RNA,RT-PCR检测LasI mRNA的表达情况,并对PAO1生物膜多糖蛋白复合物(GXL)含量进行测定。结果：①与生理盐水组比较,在干预生物膜的TE(结构熵)与生物膜厚度、抑制多糖蛋白复合物(GLX)产量以及抑制群感应系统(QS)Las 1 mRNA的表达方面,黄芩苷组、黄芩苷+黄芪甲苷组、阿奇霉素组有明显差异(P<0.01),黄芪甲苷组无明显差异(P>0.05)。②与阿奇霉素组比较,黄芩苷组在干预生物膜的结构熵(TE)与生物膜厚度以及抑制QS系统LasI mRNA的表达方面无明显差异(P>0.05),在抑制GLX产量方面弱于阿奇霉素组(P<0.05)；黄芩苷+黄芪甲苷组在干预生物膜的结构熵(TE)与生物膜厚度以及抑制QS系统LasI mRNA的表达方面弱于阿奇霉素组(P<0.01),在抑制GLX产量方面弱于阿奇霉素组(P<0.05)；黄芪甲苷组在各方面均无明显差异(P>0.05)。结论：黄芩苷在干预铜绿假单胞菌生物膜生成、减少多糖蛋白复合物产量及抑制mRNA的表达效果方面均有明显作用,而黄芪甲苷无明显作用。

Abstract

Objective:To study the effects of baicalin and astragaloside IV on the formation of Pseudomonas aeruginosa biofilm. MethodsThe Pseudomonas aeruginosa PAO1 standard strains were selected,and baicalin and astragaloside IV were used to intervene culture of PAO1 biofilm in vitro,with azithromycin as the positive control and 0.9% NaCl solution as the negative control. The morphology of biofilm under different culture conditions was observed by confocal microscopy after FITC-ConA staining. Meanwhile,the total RNA was extracted by TRIzol and the expression of LasI mRNA was detected by RT-PCR. The content of glycocallyx(GXL)in PAO1 biofilm was determined. Results:①For the comparison of the effects of intervening in biofilm TE (structure-entropy) and biofilm thickness and inhibiting GLX production and LasI mRNA expression of quorum-sensing(QS)system,there were significant differences between the normal saline group and baicalin or baicalin+astragaloside IV or azithromycin group(P<0.01),but there were no significant differences between the normal saline group and astragaloside IV group(P>0.05). ②For the comparison of the effects of intervening in biofilm TE (structure-entropy) and biofilm thickness and inhibiting LasI mRNA expression of QS system,there were no significant differences between the baicalin group and azithromycin group(P>0.05),but the effect of inhibiting GLX production in the baicalin group was weaker than that in the azithromycin group(P<0.05). For the comparison of the effects of intervening in biofilm TE (structure-entropy) and biofilm thickness and inhibiting GLX production and LasI mRNA expression of QS system,the effects in the baicalin+astragaloside IV group were weaker than those in the azithromycin group(P<0.05 or P<0.01). And there were no significant differences on these effects between the astragaloside IV group and azithromycin group(P>0.05). Conclusion:Baicalin shows the obvious effects of intervening in Pseudomonas aeruginosa biofilm formation,reducing GLX production and inhibiting LasI mRNA expression,while astragaloside IV shows no obvious effects.

关键词

铜绿假单胞菌生物膜黄芩苷黄芪甲苷

Keywords

Pseudomonas aeruginosabiofilmbaicalinAstragaloside IV

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