Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC

ZHAO Jian-ping; WU Yun; NIU Xin; ZHANG Yong-xue; XU Xing-qi; LIU Jiang-yun; HAO Li-li

您当前的位置：

首页 >

文章列表页 >

Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC

更新时间：2022-08-31

- Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC
- Shanghai Journal of Traditional Chinese Medicine Vol. 51, Issue 10, Pages: 100-102(2017)
- 作者机构：
  
  1. 上海城建职业学院健康安全系,上海,201415
  2. 苏州大学医学部药学院中药系,江苏,苏州,215123
  3. 南通卫生高等职业技术学校药学及医学技术系,江苏,南通,226000
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
ZHAO Jian-ping, WU Yun, NIU Xin, et al. Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC. [J]. Shanghai Journal of Traditional Chinese Medicine 51(10):100-102(2017)
DOI：

ZHAO Jian-ping, WU Yun, NIU Xin, et al. Content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb. by HPLC. [J]. Shanghai Journal of Traditional Chinese Medicine 51(10):100-102(2017) DOI：

摘要

目的：建立乌饭树叶中主要活性成分乌饭树苷的含量测定方法。方法：利用染色活性跟踪法,分离鉴定有效成分；采用高效液相色谱法测定其含量,色谱柱为Cosmosil-5C18 - PAQ(4.6 mm × 250 mm,5 μm),流动相为0.3%乙酸溶液乙腈梯度洗脱,流速1.0 ml/min,检测波长320 nm。结果：乌饭树苷为乌饭树叶主要有效成分,其色谱分析的分离度良好,在0.052 08 ～ 0.520 8 g/L范围内线性良好,r值为0.999 8,加样回收率为98.61%。结论：该有效成分测定方法灵敏度高,结果准确、简便,可为乌饭树叶的质量控制方法提供参考。

Abstract

Objective:To establish an HPLC method for content determination of vaccinoside in leaves of Vaccinium bracteatum Thunb.. MethodsBy tracking the dyeing activity,the active ingredients were isolated and identified. The content determination was performed by HPLC with a Cosmosil-5C18-PAQ column (4.6 mm×250 mm,5 μm). The mobile phase was 0.3% acetic acid- acetonitrile with gradient elution at a flow rate of 1.0 ml/min. The wavelength of detection was 320 nm. Results:Vaccinoside was separated and identified as the main active ingredient. The chromatographic analysis showed a good separation. There was a good linearity in the range of 0.052 08-0.520 8 g/L(r=0.999 8). The recovery rate was 98.61%. Conclusion:The determination method is quick and accurate,which could provide reference for the quality control of vaccinoside in leaves of Vaccinium bracteatum Thunb..

关键词

乌饭树乌饭树苷HPLC质量控制

Keywords

Vaccinium bracteatum Thunb.vaccinosideHPLCquality control

references

Views

186

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Study on quality control methods and bacteriostatic effects of Xiakucao (Prunellae Spica)-Wubeizi (Acanthopanacis Cortex) bacteriostasis lotion

Comparison of characteristic processing between Menghe medical school’s clinical prescriptions and Guide on Preparation of Drugs

Research advance on chemical constituents, pharmacological action and quality control of Radix Codonopsis

Quality control of Erkeling syrup

Technology and quality control for processing Fructus Aurantii Immaturus simulating ancient method in classic recipe wendan decoction

Related Author

No data

Related Institution

School of Pharmacy,Shanghai University of Traditional Chinese Medicine

Changzhou Hospital of Traditional Chinese Medicine

Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine

The MOE Key Laboratory for Standardization of Chinese Medicines

Department of Pharmacy,Lianyungang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine

⁰