Objective:To study the characteristics of mice model with deficiency syndrome induced by hydrocortisone and the potential material base. Methods 60 male Kunming mice were randomly divided into the normal control group and model group,30 mice in each group. The mice in the normal control group were treated with normal water and feeding,and the mice in the model group were treated with hydrocortisone solution with degressive dose(0.015 g·kg-1·d-1 for 3 days,0.010 g·kg-1·d-1 for 5 days and 0.007 5 g·kg-1·d-1 for 7 days). On the eighth and fifteenth day,the syndrome classification of mice was determined by standard acquisition technique and syndrome differentiation methods for the four methods of diagnosis in mice that created by our research group. On the third,eighth and fifteenth day,the mice were sacrificed respectively. The adrenal gland was collected and the RNA and protein were extracted. The expressions of related genes were detected by real time fluorescent quantitative PCR,the expression of steroidogenic acute regulatory protein(STAR) was detected by Western blotting,and the content of serum adrenocorticotropic hormone (ACTH) was measured by ELISA.  Results: ①Compared with the normal control group,the body weight of the mice in the model group was significantly decreased during the modeling period(P<0.05),and the appearance such as marasmus,fluffy hair falling,decreased glossiness of hair,reduced activity with curled posture were observed,the temperature especially in the tail was decreased,but there was no obvious change on the claw color. ②Compared with the normal control group on the fifteenth day,the mice in the model group showed obvious syndrome of qi deficiency and yin deficiency,the persistent atrophy of adrenal gland,spleen and thymus were observed(P<0.05). The serum level of ATCH was decreased in the model group(P<0.05),the gene expressions of Srb1 and Ldlr as the important receptor in adrenocortical hormone synthesis were decreased(P<0.01),the gene expressions of steroid hormone synthases such as Star,Cyp11a1,Cyp21a1,Cyp11b1,Cyp11b2 and their transcription factors such as Fdx1,Fdxr,Nr4a1,Nr4a2 and Nr5a1 were significantly decreased (P<0.05), and the protein expression level of StAR was significantly decreased(P<0.01).  Conclusion: Hydrocortisone can be used to induce the mice model with deficiency syndrome,which shows the characteristics of syndrome of qi deficiency and yin deficiency. The important functional molecules in the adrenocortical hormone synthesis are associated with the related syndrome.