Protective effects of Xiaochaihu Decoction and Danggui Shaoyao Powder with single and combined application on immunological liver injury

SONG Wu; WEI Lin; LIU Zhi; WANG Xiao-yan; ZHANG Chi; JIANG Shuang

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Protective effects of Xiaochaihu Decoction and Danggui Shaoyao Powder with single and combined application on immunological liver injury

更新时间：2022-08-31

- Protective effects of Xiaochaihu Decoction and Danggui Shaoyao Powder with single and combined application on immunological liver injury
- Shanghai Journal of Traditional Chinese Medicine Vol. 51, Issue 6, Pages: 88-92(2017)
- 作者机构：
  
  1. 长春中医药大学基础医学院,吉林,长春,130117
  2. 吉林省中医药科学院第一临床医院药剂科,吉林,长春,130021
- 作者简介：
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SONG Wu, WEI Lin, LIU Zhi, et al. Protective effects of Xiaochaihu Decoction and Danggui Shaoyao Powder with single and combined application on immunological liver injury. [J]. Shanghai Journal of Traditional Chinese Medicine 51(6):88-92(2017)
DOI：

SONG Wu, WEI Lin, LIU Zhi, et al. Protective effects of Xiaochaihu Decoction and Danggui Shaoyao Powder with single and combined application on immunological liver injury. [J]. Shanghai Journal of Traditional Chinese Medicine 51(6):88-92(2017) DOI：

摘要

目的：考察小柴胡汤和当归芍药散单方及合用对免疫性肝损伤的保护作用。方法：昆明小鼠72只随机分为6组,空白组、模型组、小柴胡汤组(6.63 g/kg)、当归芍药散组(11.31 g/kg)、小柴胡汤合当归芍药散(柴归汤)组(17.94 g/kg)和阳性药(联苯双脂合甘草酸二铵)组(0.2 μg/g,0.3 μg/g)。除空白组外,其余各组采用刀豆蛋白A(Con A)尾静脉注射制造免疫性肝损伤模型,各组采取相应干预7天。采用动物半自动生化分析仪检测ALT、AST、ALB和TP水平；HE染色观察肝组织病理切片；比色法检测肝组织SOD活性、MDA和GSH含量；ELISA法检测TNF-α、IL-1和IL-6；Western blotting法检测Bcl-2、Bax和Caspase-3蛋白表达；APAAP法检测T淋巴细胞亚群。结果：与模型组比较,小柴胡汤组可降低ALT和AST水平,减少IL-1,降低MDA含量,显著降低Bcl-2蛋白表达；当归芍药散组可降低ALT水平,降低血清TNF-α、IL-1和IL-6炎症因子,并降低Bcl-2蛋白含量；小柴胡汤合当归芍药散对上述指标均有显著保护作用,对各指标改善优于单方,且能显著改善血清CD4,+,/CD8,+,比值。结论：小柴胡汤和当归芍药散均能够保护刀豆蛋白A所致免疫性肝损伤,两方合用优于单方,其机制与提高抗氧化、抗凋亡能力及调节免疫失调多方面作用有关。

Abstract

Objective:To investigate the protective effects of Xiaochaihu Decoction and Danggui Shaoyao Powder with single and combined application on immunological liver injury. Methods Seventy-two KM mice were randomly divided into the blank group, model group, Xiaochaihu Decoction(XCHD,6.63 g/kg) group, Danggui Shaoyao Powder(DGSYP,11.31 g/kg)group, combination(Chaigui Decoction,17.94 g/kg)group and positive control group (bifendate 0.2 μg/g and diammonium glycyrrhizinate 0.3 μg/g). Except the blank group, the model of immunological liver injury was established in the other group by intravenous injection of concanavalin A(Con A)in mouse tail. Each group was treated with the corresponding intervention for 7 days. The levels of ALT, AST, ALB and TP were detected by semi-automatic biochemical analyzer. The pathological section was observed by HE staining. The activity of SOD and the contents of GSH and MDA in liver tissue were determined by colorimetry. The levels of TNF-α,IL-1 and IL-6 were detected by ELISA. The protein expressions of Bax, Bcl-2 and Caspase-3 were detected by Western blotting. The T lymphocyte subsets were detected by APAAP method. Results: Compared with the model group, XCHD could decrease the ALT and AST levels, reduce the IL-1 level, decrease the MDA content, and Chaigui Decoction significantly decrease the Bcl-2 protein expression. Compared with the model group, DGSYP could decrease the ALT level, reduce the serum levels of TNF-α,IL-1 and IL-6, and reduce the protein content of Bcl-2. Chaigui Decoction showed significantly protective effects. The effects on improving above indicators in the combination group were better than those in the XCHD group and DGSYP group,and Chaigui Decoction significantly improved the ratio of CD4,+,/CD8,+, in serum. Conclusion: Xiaochaihu Decoction and Danggui Shaoyao Powder show the protective effects on ConA induced immunological liver injury respectively. The combination of the two prescriptions shows better effects than the single application. The mechanism may be associated with promotion on anti-oxidation,anti-apoptosis and regulation of immune disorders.

关键词

小柴胡汤当归芍药散免疫性肝损伤刀豆蛋白A

Keywords

Xiaochaihu DecoctionDanggui Shaoyao Powderimmunological liver injuryconcanavalin A

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