Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer

WANG Qing; QUE Zu-jun; LUO Bin; ZHENG Ting-ting; TIAN Jian-hui

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Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer

更新时间：2022-08-31

- Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer
- Shanghai Journal of Traditional Chinese Medicine Vol. 51, Issue 5, Pages: 77-81(2017)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院中医肿瘤研究所,上海,200032
  2. 上海市中医药研究院中医肿瘤研究所,上海,200032
- 作者简介：
- 基金信息：
- DOI：
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WANG Qing, QUE Zu-jun, LUO Bin, et al. Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer. [J]. Shanghai Journal of Traditional Chinese Medicine 51(5):77-81(2017)
DOI：

WANG Qing, QUE Zu-jun, LUO Bin, et al. Effects of Polyphyllin I on apoptosis and cycle of circulating tumor cells of lung cancer. [J]. Shanghai Journal of Traditional Chinese Medicine 51(5):77-81(2017) DOI：

摘要

目的：通过体外药效评价重楼皂苷Ⅰ对肺癌循环肿瘤细胞(circulating tumor cells,CTC)增殖、凋亡的影响。方法：分别用不同浓度的重楼皂苷Ⅰ干预CTC细胞24 h、48 h、72 h后,采用CCK-8法检测细胞增殖情况,采用流式细胞术和激光共聚焦显微镜分析CTC凋亡与周期情况。结果：重楼皂苷Ⅰ能够显著抑制CTC增殖并具有浓度依赖性,诱导CTC细胞核形态发生明显改变,使凋亡细胞的比例显著升高,并将CTC细胞的周期阻滞在G0/G1期。结论：重楼皂苷Ⅰ通过诱导CTC凋亡,抑制细胞从G0/G1期向S期转化,从而发挥抗肿瘤作用。

Abstract

Objective:To evaluate the effects of Polyphyllin I on proliferation and apoptosis of circulating tumor cells (CTC) of lung cancer in vitro. MethodsThe CTC were intervened by Polyphyllin I at different concentrations for 24,8 and 72 hours. The cell proliferation was determined by CCK-8,and the cell apoptosis and cycle were detected by flow cytometry and confocal laser scanning microscopy. Results:Polyphyllin I could significantly inhibit the CTC proliferation in a concentration-dependent manner,and induce the significant morphological changes of CTC nucleus. The proportion of apoptotic cells was significantly increased,and the cells cycle was stagnated in G0/G1 phase. Conclusion:Polyphyllin I can induce CTC apoptosis and inhibit the cells differentiation from G0/G1 to S phase,which plays an anti-tumor effects.

关键词

重楼皂苷Ⅰ肺癌循环肿瘤细胞细胞增殖细胞凋亡细胞周期

Keywords

polyphyllin Icirculating tumor cells of lung cancercell proliferationcell apoptosiscell cycle

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