Preliminary mechanism research of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox

ZOU Yu; YUAN Yu-xia; HE Xue; TANG Xue-Yao; ZHANG Yi-Xi; QIU Yan-yan; YUAN Ze-ting; XU Ke; YIN Pei-hao

您当前的位置：

首页 >

文章列表页 >

Preliminary mechanism research of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox

更新时间：2022-08-31

- Preliminary mechanism research of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox
- Shanghai Journal of Traditional Chinese Medicine Vol. 51, Issue 4, Pages: 90-94(2017)
- 作者机构：
  
  1. 上海中医药大学附属普陀医院普外科,上海,200062
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
ZOU Yu, YUAN Yu-xia, HE Xue, et al. Preliminary mechanism research of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox. [J]. Shanghai Journal of Traditional Chinese Medicine 51(4):90-94(2017)
DOI：

ZOU Yu, YUAN Yu-xia, HE Xue, et al. Preliminary mechanism research of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox. [J]. Shanghai Journal of Traditional Chinese Medicine 51(4):90-94(2017) DOI：

摘要

目的：初步探讨六神丸逆转人结肠癌耐阿霉素细胞株Lovo/Dox的机制。方法：设置对照组、阿霉素组(Dox)、六神丸组和阿霉素与六神丸联合用药组。用Cell Counting Kit-8(CCK-8)法检测六神丸对人结肠癌耐药细胞株(Lovo/Dox、HCT116/L-OHP、Caco,2,/Dox)的影响；蛋白质免疫印迹法(Western Blotting)检测耐药蛋白P-gp、BCRP以及相关凋亡蛋白caspase-3、caspase-9、Cytochrome C和抑癌蛋白p53在人结肠癌Lovo/Dox细胞中的表达；免疫荧光实验检测P-gp的荧光表达。结果：六神丸作用于人结肠癌3种耐药细胞24 h后,CCK-8结果显示其可抑制3种人结肠癌细胞株的活性,其中抑制人结肠癌耐阿霉素细胞株Lovo/Dox的效果最明显。六神丸联合阿霉素可上调caspase-3、caspase-9、p53蛋白表达水平以及减弱P-gp蛋白及荧光表达。结论：六神丸可逆转人结肠癌耐阿霉素细胞株Lovo/Dox的P-gp表达,可能是通过激活相关凋亡途径和抑癌蛋白而引起该细胞株凋亡。

Abstract

Objective:To preliminarily discuss the mechanism of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox. MethodsThe control group,doxorubicin group,Liushen Pill group and combination group were divided. The effects of Liushen Pill on drug-resistant human colon cancer cell lines (Lovo/Dox,HCT116/L-OHP,Caco,2,/Dox)were detected by Cell Counting Kit-8(CCK-8). The expressions of P-gp, BCRP and related apoptosis proteins caspase-3, caspase-9, Cytochrome C and tumor suppressor protein p53 in human colon cancer cell line Lovo/Dox were determined by Western blot. The fluorescence expression of P-gp was detected by immunofluorescence assay. Results:After the intervention of Liushen Pill for 24 hours,the CCK-8 showed that Liushen Pill could inhibit the activities of the three drug-resistant human colon cancer cell lines,among the inhibiting effects on doxorubicin resistant human colon cancer cell line Lovo/Dox were most significant. The treatment of Liushen Pill combined with doxorubicin could up-regulate the protein expression of caspase-3,caspase-9 and p53 and decrease the protein and fluorescence expression of P-gp. Conclusion:Liushen Pill can reverse the expression of P-gp in doxorubicin resistant human colon cancer cell line Lovo/Dox, which may be related to the activation of the related apoptosis pathway and tumor suppressor protein.

关键词

人结肠癌细胞株六神丸凋亡机制蛋白表达阿霉素耐药逆转

Keywords

human colon cancer cell lineLiushen Pillapoptosis mechanismprotein expressiondoxorubicinreversing drug-resistance

references

Views

144

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Sijunzi Decoction and Siwu Decoction on expression of EGF, EGF mRNA, VEGFA and VEGF mRNA in cultured human skin fibroblasts in vitro

Research progress on prevention of anthracycline-induced cardiotoxicity by Chinese medicine

Related Author

No data

Related Institution

Surgery of Traditional Chinese Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine

Department of Mastopathy, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine

⁰