Objective:To observe the influence of five mimic-animal exercise on knee-joint muscle strength and balance capacity in middle-aged and elderly women. Methods 56 middle-aged and elderly women volunteers were divided into the intervention group and control group by the methods of quasi-experiment,28 cases in each group. Five mimic-animal exercise were performed in the intervention group and stretching exercises were performed in the control group,with a course of 12 months. The indexes of isokinetic test in knee-joint and the scores of Berg balance scale were observed and compared.  Results:①Comparison of different time points(T1 refers to treatment before,T2 and T3 refer to treatment for 6 and 12 months respectively)in the intervention group:For the comparison of peak torque/body weight(PW)in right knee extensors and left knee flexors,there were statistically significant differences between T1 and T2 (P<0.05)；for the PW comparison in extensors and flexors of left and right knee,there were no statistically significant differences between T2 and T3(P>0.05),there were statistically significant differences between T1 and T3(P<0.05).Comparison of different time points in the control group:for the PW comparison in extensors and flexors of left and right knee,there were no statistically significant differences between T1 and T2,T2 and T3,T1 and T3(P>0.05).②For the comparison in extensors of left and right knee at T3, there was statistically significant difference on PW between the two groups(P<0.05)；while for the comparison in flexors of left and right knee at T3,there was no statistically significant difference on PW between the two groups(P>0.05).③Comparison of different time points in the intervention group:there were statistically significant differences on Berg scores between T1 and T2,T2 and T3,T1 and T3(P<0.05). Comparison of different time points in the control group:there were statistically significant differences on Berg scores between T1 and T2,T1 and T3(P<0.05),but there was no statistically significant difference on Berg score between T2 and T3(P>0.05).There was statistically significant difference on Berg scores between the two groups at T3(P<0.05).  Conclusion:Five mimic-animal exercise can improve the knee-joint muscle strength in middle-aged and elderly women,which slow down the muscle weakness and improve the balance capacity.