Objective:To observe the effects of Qinggan Huatan Huoxue Decoction on the expressions of aquaglyceroporin-7(AQP-7)and -9(AQP-9)in nonalcoholic steatohepatitis(NASH)rats. Methods Rats model was copied by the feeding of high fat diet. On the 8th week,5 rats in the normal group and 10 rats in the model group were sacrificed respectively. Then the remaining rats in the model group were divided into the high fat diet group,Yishanfu Tablet group and Qinggan Huatan Huoxue Decoction group. After administration for 5 weeks,the rats were sacrificed and the serum and liver tissue samples were collected. The serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triacylglycerol (TG), cholesterol (TC) and the expression of AQP-7,9 in liver tissue were detected. The pathological changes of liver tissues were observed by HE staining under light microscope.  Results: On 8th week, there were significant differences on the degree of steatosis and score of inflammatory activity between the model group and normal group(P<0.01). On 13th week, there were significant differences on the degree of steatosis and score of inflammatory activity between the high fat diet group and normal group,western medicine group,Chinese medicine group(P<0.05,P<0.01). There were significant differences on the levels of AQP-7 and AQP-9 mRNA between the high fat diet group and normal group,western medicine group,Chinese medicine group(P<0.01). There were statistically significant differences on the degree of steatosis and score of inflammatory activity between the high fat diet group and Qinggan Huatan Huoxue Decoction group(P<0.05).Compared with the high fat diet group,the contents of AST, ALT, TG, TC in the Qinggan Huatan Huoxue Decoction group were significantly decreased(P<0.01),the level of AQP-7 in liver tissue was significantly increased(P<0.01)and the level of AQP-9 was significantly decreased(P<0.01).  Conclusion: AQP-7,9 are associated with the pathogenesis of nonalcoholic steatohepatitis. Qinggan Huatan Huoxue Decoction may regulate the expression of AQP-7,9 and improve lipid metabolism of liver cells,which plays a role in the prevention and treatment of nonalcoholic steatohepatitis.