Objective:To study the inhibitive effects of Lingmao Formula on cccDNA, pgRNA, sRNA and La-mRNA in HepG2.2.15 and HepAD38 cells, and discuss its mechanism on anti-hepatitis B virus(HBV). MethodsThe aqueous extract of Lingmao Formula was prepared. HepG2.2.15 and HepAD38 cells were intervened by the aqueous extract of Lingmao Formula at concentration of 500 and 1 000 mg/L respectively,the blank control group was treated with normal saline. The supernatant of culture medium was collected after 6 days and the levels of HBsAg and HBeAg were detected by ELISA. The cells were collected and the total RNA was extracted,the expression levels of cccDNA, pgRNA, sRNA and La mRNA were detected by RT-PCR.  Results:Compared with the blank control group, the secretion levels of HBsAg and HBeAg in HepG2.2.15 and HepAD38 cells of the Lingmao Formula group were significantly decreased, the expression levels of intracellular cccDNA, pgRNA, sRNA and La mRNA were significantly decreased (P< 0.01).  Conclusion:Lingmao Formula may promote the degradation of pgRNA, sRNA and cccDNA by inhibiting La protein expression in HepG2.2.15 and HepAD38 cells, and result in the inhibition of HBV replication.