Objective:To optimize the extraction process of total flavonoids from Anchusa italica Retz. . Methods According to the extraction efficiency of total flavonoids and rutin and the extraction ratio, the process of refluxing extraction of Anchusa italica Retz. was optimized by two kinds of multi-index comprehensive score method and L,9,(3,4,) repeated orthogonal design.  Results:The methods of content determination for total flavonoids and rutin in the extracts were accordance with the requirements of methodology. The optimized refluxing extraction process of total flavonoids from Anchusa italica Retz. as follows:the ratio of material and liquid is 1∶20(w/v), extraction temperature is 100 ℃,the volume fraction of ethanol is 75%,twice extraction and each 2 hours.  Conclusion: The validation test shows that the optimized extraction process is simple,reasonable and feasible,which provides the theory basis for the subsequent studies.