Simultaneous determination of five triterpenoid saponins in Bacopa monnieri extract by quantitative analysis of multi-components with a single marker

CUI Yu-qing; ZHANG Ji-quan; WU Fei; RUAN Ke-feng

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Simultaneous determination of five triterpenoid saponins in Bacopa monnieri extract by quantitative analysis of multi-components with a single marker

更新时间：2022-08-31

- Simultaneous determination of five triterpenoid saponins in Bacopa monnieri extract by quantitative analysis of multi-components with a single marker
- Shanghai Journal of Traditional Chinese Medicine Vol. 50, Issue 7, Pages: 88-93(2016)
- 作者机构：
  
  1. 上海中医药大学中药现代制剂技术教育部工程研究中心,上海,201203
  2. 上海张江中药现代制剂技术工程研究中心,上海,201203
  3. 上海中医药大学中药学院,上海,201203
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CUI Yu-qing, ZHANG Ji-quan, WU Fei, et al. Simultaneous determination of five triterpenoid saponins in Bacopa monnieri extract by quantitative analysis of multi-components with a single marker. [J]. Shanghai Journal of Traditional Chinese Medicine 50(7):88-93(2016)
DOI：

CUI Yu-qing, ZHANG Ji-quan, WU Fei, et al. Simultaneous determination of five triterpenoid saponins in Bacopa monnieri extract by quantitative analysis of multi-components with a single marker. [J]. Shanghai Journal of Traditional Chinese Medicine 50(7):88-93(2016) DOI：

摘要

目的：利用一测多评法建立假马齿苋中间体中5种三萜皂苷类成分的高效液相色谱分析方法。方法：以BacopasideⅠ为内参物,建立Bacoside A,3,、BacopasideⅡ、Bacopasaponin C isomer、Bacopasaponin C的相对校正因子,从而计算各成分含量,并将一测多评结果与外标法实测值用相对误差评价,验证一测多评法的准确性。结果：BacopasideⅠ与Bacoside A,3,、BacopasideⅡ、Bacopasaponin C isomer、Bacopasaponin C的相对校正因子分别为0.980、0.747、1.046、0.836,一测多评法计算结果与外标法无显著差异,相对误差小于5%。结论：该方法准确可靠,简便可行,可作为假马齿苋中间体多指标成分测定质量控制方法。

Abstract

Objective:To establish a quantitative analysis of multi-components by single-marker (QAMS) HPLC method for the determination of five triterpenoid saponins in the intermediates of Bacopa monnieri. Methods BacopasideⅠwas used as the internal marker to establish relative correction factor (RCF) between BacopasideⅠand other four triterpenoid saponins, and RCF was used in the calculation of their content. Relative error was used in the appraisal of the result between external standard method and QAMS. Results: The RCF between BacopasideⅠand other four triterpenoid saponins were 0.980,0.747,1.046 and 0.836, respectively. The two methods did not show significant difference in assay results and the RCFs were reliable with the relative error less than 5%. Conclusion: The established QAMS method is feasible, accurate, simple and can be used as a method for the determination of multi-component index in the extract of Bacopa monnieri.

关键词

假马齿苋一测多评法相对校正因子外标法假马齿苋皂苷Ⅰ苦艾素A3假马齿苋皂苷Ⅱ假马齿苋皂苷C异构体假马齿苋皂苷C

Keywords

Bacopa monnieriquantitative analysis of multi-assessment by single marker (QAMS)relative correction factor (RCF)external standard method (ESM)BacopasideⅠBacoside A3BacopasideⅡBacopasaponin C isomerBacopasaponin C

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Institute of Agricultural Modernization, Jilin Agricultural University

College of Chinese Medicinal Material, Jilin Agricultural University

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