Study on mechanism of IL-6/STAT3 signaling pathway in ulcerative colitis and intervention by “Xianglian Pill”

DONG Yan; CAO Yong-qing; LU Jin-gen

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Study on mechanism of IL-6/STAT3 signaling pathway in ulcerative colitis and intervention by “Xianglian Pill”

更新时间：2022-08-31

- Study on mechanism of IL-6/STAT3 signaling pathway in ulcerative colitis and intervention by “Xianglian Pill”
- Shanghai Journal of Traditional Chinese Medicine Vol. 50, Issue 6, Pages: 75-79(2016)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院中医外科研究所,上海,200032
  2. 上海中医药大学附属龙华医院肛肠科,上海,200032
- 作者简介：
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DONG Yan, CAO Yong-qing, LU Jin-gen. Study on mechanism of IL-6/STAT3 signaling pathway in ulcerative colitis and intervention by “Xianglian Pill”. [J]. Shanghai Journal of Traditional Chinese Medicine 50(6):75-79(2016)
DOI：

DONG Yan, CAO Yong-qing, LU Jin-gen. Study on mechanism of IL-6/STAT3 signaling pathway in ulcerative colitis and intervention by “Xianglian Pill”. [J]. Shanghai Journal of Traditional Chinese Medicine 50(6):75-79(2016) DOI：

摘要

目的：通过STAT3抑制剂及香连丸的干预,观察溃疡性结肠炎(UC)模型大鼠结肠组织IL-6、STAT3蛋白变化,探讨UC发病中IL-6/STAT3信号通路的变化机制。方法：将100只雄性Wistar大鼠随机分为正常组、模型组、中药组(香连丸)、抑制剂组、中药+抑制剂组。采用经典TNBS灌肠法复制UC大鼠模型,造模成功后予抑制剂AG-490腹腔注射及香连丸灌胃干预。观察大鼠结肠黏膜组织损伤程度并评分,透射电镜观察各组结肠黏膜情况,Western blotting检测IL-6、STAT3蛋白变化。结果：UC模型大鼠结肠部位溃疡形成,肉眼及镜下观察可见炎症、充血、水肿等病理改变。与正常组比较,UC模型大鼠结肠组织IL-6、STAT3蛋白表达均显著增高(P<0.001)；与模型组比较,中药组、抑制剂组及中药+抑制剂组结肠组织中IL-6、STAT3蛋白表达水平相对降低(P<0.001)。结论：IL-6/STAT3通路相关因子表达异常是溃疡性结肠炎发生的重要分子生物学基础,香连丸可通过调节IL-6/STAT3通路抑制UC肠黏膜STAT3的活化,促进炎症细胞凋亡,从而起到临床治疗作用。

Abstract

Objective:This study aims to investigate the pathogenesis of ulcerative colitis (UC). The changes of IL-6 and STAT3 protein in colon of UC model rats were observed after intervention with STAT3 pathway inhibitor or/and by “Xianglian Pill”. MethodsOne hundred male Wistar rats were randomly divided into 5 groups:control group, model group, Chinese medicine group(“Xianglian Pill”), inhibitor group, and Chinese medicine plus inhibitor group. The experiment was performed in rats of UC induced by TNBS enema, and then AG-490 and “Xianglian Pill” were administered to rats by intraperitoneal injection and gavage respectively. Colonic mucosal tissue were observed and evaluated by light and electron microscopy. The expressions of IL-6 and STAT3 protein were tested by western blot. Results:There were many ulcers in the colon of UC model rats, the pathological changes in colonic mucosa such as inflammation, congestion and edema were observed in the colon of UC model rats by naked eye and microscope. The expressions of IL-6 protein and STAT3 protein were increased in the colon of UC model rats, while decreased in Chinese medicine group, inhibitor group and Chinese medicine plus inhibitor group. Conclusion: The abnormal expression of related factors in IL-6/STAT3 pathway is the molecular basis for the occurrence of ulcerative colitis. “Xianglian Pill” can regulate the IL-6/STAT3 pathway to inhibit activation of STAT3 in UC intestinal mucosa, and promote the apoptosis of inflammatory cells.

关键词

溃疡性结肠炎香连丸IL-6/STAT3信号通路

Keywords

ulcerative colitis“Xianglian Pill”IL-6/STAT3 signaling pathway

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