Objective:To study the effects of “Chang Ji Tai” on 5-HT2A receptor (5-HT2AR), 5-HT,7, receptor (5-HT,7,R) and transient receptor potential vanilloid type-1(TRPV1) of dorsal root ganglia in irritable bowel syndrome(IBS) rats of visceral hypersensitivity. MethodsSixty neonatal Sprague-Dawley rats were randomly divided into 6 groups:normal control group, model control group, positive control group, “Chang Ji Tai” low-dose group, middle-dose group and high-dose group. The IBS rats model of visceral hypersensitivity was induced by intrarectal acetic acid irritation in the latter 5 groups. During the period of making model capsazepine (TRPV1 antagonist) was intraperitoneally injected (2 μg/g) in positive control group 30 min before intrarectal irritation. Four weeks after the end of modeling, “Chang Ji Tai” with concentrations of respectively 2.5 g/kg, 5 g/kg and 10 g/kg were administered intragastrically every day in “Chang Ji Tai” each dose group respectively, while equivalent deionized water was administered intragastrically in the other three groups. After 4-week treatment, rectal balloon distension was applied to evaluate visceral hypersensitivity by score of abdominal withdrawal reflex (AWR). The levels of 5-HT2AR, 5-HT,7,R and TRPV1 in dorsal root ganglia were detected by immunohistochemistry.  Results:Compared with the normal control group, the scores of AWR with the 40,0 and 80 mmHg pressure were significantly higher in model control group (P,<,0.01). Compared with the model control group, the scores of AWR with the 40 and 60 mmHg pressure were significantly lower in all “Chang Ji Tai” groups (P,<,0.05, P,<,0.01). The expressions of 5-HT2AR, 5-HT,7,R and TRPV1 of dorsal root ganglia in model control group were significantly higher than that in the normal control group (P,<,0.01). The expressions of 5-HT2AR, 5-HT,7,R and TRPV1 of dorsal root ganglia in all “Chang Ji Tai” groups were significantly lower than that in model control group (P,<,0.01). There was no significant difference between “Chang Ji Tai” high-dose group and normal control group in the levels of 5-HT2AR, 5-HT,7,R and TRPV1 of dorsal root ganglia (P,>,0.05).  Conclusion: “Chang Ji Tai” can reduce the visceral hypersensitivity by regulating the expressions of 5-HT2AR, 5-HT,7,R and TRPV1 of dorsal root ganglia.