Effects of Saikosaponin d on mitochondrial membrane potential and apoptosis in human neuroblastoma SH-SY5Y cells

LI Yan; ZHAO Dong-hai; ZHANG Wei; LUO Jun; ZHU Wen-he; LYU Shi-jie

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Effects of Saikosaponin d on mitochondrial membrane potential and apoptosis in human neuroblastoma SH-SY5Y cells

更新时间：2022-08-31

- Effects of Saikosaponin d on mitochondrial membrane potential and apoptosis in human neuroblastoma SH-SY5Y cells
- Shanghai Journal of Traditional Chinese Medicine Vol. 50, Issue 6, Pages: 90-93(2016)
- 作者机构：
  
  1. 吉林医药学院生物化学与分子生物学教研室,吉林,吉林,132013
  2. 吉林医药学院病理学教研室,吉林,吉林,132013
- 作者简介：
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LI Yan, ZHAO Dong-hai, ZHANG Wei, et al. Effects of Saikosaponin d on mitochondrial membrane potential and apoptosis in human neuroblastoma SH-SY5Y cells. [J]. Shanghai Journal of Traditional Chinese Medicine 50(6):90-93(2016)
DOI：

LI Yan, ZHAO Dong-hai, ZHANG Wei, et al. Effects of Saikosaponin d on mitochondrial membrane potential and apoptosis in human neuroblastoma SH-SY5Y cells. [J]. Shanghai Journal of Traditional Chinese Medicine 50(6):90-93(2016) DOI：

摘要

目的：研究不同浓度的柴胡皂苷d (saikosaponin d,SSd) 对人神经母细胞瘤SH-SY5Y细胞线粒体膜电位(mitochondrial transmembrane potential,△ψm)及凋亡水平的影响。方法：体外常规培养SH-SY5Y细胞,将细胞置于分别含0~10 μmol/L SSd的DMEM高糖培养基中,作用48 h；Rh123染色后流式细胞仪检测细胞线粒体膜电位变化,Tunel法、吖啶橙(AO)/溴化乙啶(EB)染色法检测细胞凋亡水平的变化。结果：与对照组比较,8~10 μmol/L SSd 预处理的SH-SY5Y 细胞TUNEL染色后凋亡指数明显升高(P<0.01)；AO/EB染色后可见明显的阳性细胞。此外,不同浓度的SSd作用后均可见SH-SY5Y 细胞中Rh123荧光强度减弱,且细胞Rh123荧光强度降低有剂量依赖关系。结论：一定浓度的SSd可引起 SH-SY5Y 细胞凋亡,其机制可能与影响线粒体膜电位有关。

Abstract

Objective:To investigate the effects of Saikosaponin d (SSd) on mitochondrial transmembrane potential (△ψm) and apoptosis in human neuroblastoma cells (SH-SY5Y). MethodsSH-SY5Y cells were cultured in high glucose DMEM; and SSd was dissolved in DMSO. SH-SY5Y cells were co-cultured with 0~10 μmol/L SSd for 48h. the △ψm of SH-SY5Y cells were analyzed with flow cytometry by Rh123 staining. At the same time, apoptosis levels of cells were analyzed by acridine orange (AO)/ethidium bromide (EB) staining and TUNEL Kit. Results:The fluorescence intensity of Rh123 was reduced significantly in SH-SY5Y cells after treatment with SSd for 48 h. It also showed that 8-10 μmol/L of SSd induced the increase of apoptotic index of SH-SY5Y cells by TUNEL staining (P<0.01). Conclusion: SSd can promote the apoptosis of SH-SY5Y cells by reducing △ψm.

关键词

柴胡皂苷dSH-SY5Y细胞线粒体膜电位凋亡

Keywords

saikosaponin dSH-SY5Y cells△ψmapoptosis

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