Objective:To Study on the differentially expressed miRNA of drug-contained serum of “Bushen Decoction” on rat intervertebral disc chondrocytes, which aims to provide target miRNA for treating cervical spondylosis with “Bushen Decoction” , and to discuss the function mechanism by regulating the target of related genes. MethodsThe primary rat intervertebral disc chondrocytes were identified by collagen type Ⅱ immunofluorescent staining and tuluidine blue staining. The intervertebral disc chondrocytes were cultured in serum containing “Bushen Decoction” or blank serum respectively. After a given time of intervention,the cultured cells were collected,and the total RNA were isolated and purified and used for follow-up miRNA microarray experiments. Real-time quantitative PCR was used to further validate the expression levels over 4-fold up-regulated.  Results:The twice-enzyme digestion method was practicable,efficient and ideal technique to obtain a large number of purified primary rat intervertebral disc chondrocytes in a short time and culture cells with typical intervertebral disc chondrocytes characteristics. Collagen type Ⅱ immunofluorescent staining and tuluidine blue staining were positive. The miRNA expression profiles in “Bushen Decoction” containing serum groups compared inner the groups after comparing with that from blank serum control subjects. Compared with drug-contained serum of “Bushen Decoction” on rat intervertebral disc chondrocytes cultrured 1d, 121 over 2-fold different expression of miRNA (53 up-regulated and 68 down-regulated) were identified in serum cultured 3d. The results of PCR were in accordance with the miRNA array results that the expression levels over 4-fold up-regulated.  Conclusion:A set of specific miRNA were obtained after “Bushen Decoction” containing serum intervention on rat intervertebral disc chondrocytes for 3d. Target gene prediction software to predict the up-regulated miRNA mainly by improving the nutritional metabolism of intervertebral disc through the target genes such as Opn,Col,Bcl212,Vdr,IGF-I and Mmp-13,et al.