The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4<sup>+</sup> CD25<sup>+</sup> Foxp3T/Th17 cells

LU Yan; ZHANG Qi-chun; WANG Yue; ZHU Hao-han

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The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4⁺ CD25⁺ Foxp3T/Th17 cells

更新时间：2022-08-31

- The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4⁺ CD25⁺ Foxp3T/Th17 cells
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 12, Pages: 68-72(2015)
- 作者机构：
  
  1. 江苏省中医院风湿免疫科,江苏,南京,210029
  2. 南京中医药大学,江苏,南京,210046
- 作者简介：
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LU Yan, ZHANG Qi-chun, WANG Yue, et al. The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4⁺ CD25⁺ Foxp3T/Th17 cells. [J]. Shanghai Journal of Traditional Chinese Medicine 49(12):68-72(2015)
DOI：

LU Yan, ZHANG Qi-chun, WANG Yue, et al. The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4⁺ CD25⁺ Foxp3T/Th17 cells. [J]. Shanghai Journal of Traditional Chinese Medicine 49(12):68-72(2015) DOI：

摘要

目的：研究生津润燥方对免疫诱导干燥综合征模型小鼠外周血CD4,+,CD25,+, Foxp3T细胞(Treg细胞)及Th17细胞水平的影响,同时观察腺体受累的改善状况和外周血淋巴细胞亚群的变化。方法：雄性BABL/C小鼠分为空白组,模型组,硫酸羟氯喹组,生津润燥方高、中、低剂量组。除空白组外,其余各组小鼠采用免疫诱导法建立干燥综合征小鼠模型。各组采取相应干预措施。采用流式细胞术检测模型小鼠外周血中CD4,+,、CD8,+,、CD4,+,CD25,+, Foxp3T细胞及Th17细胞水平,并测定模型鼠唾液、泪液分泌量；治疗的末期,观察下颌腺的组织形态学改变。结果：与空白组比较,模型组第1周到第4周小鼠唾液和泪液流量均显著降低(P,<,0.05)；与模型组比较,生津润燥方各剂量组第2、3、4周小鼠唾液流量和泪流量升高(P,<,0.05)。与空白组比较,模型组Th17淋巴细胞水平升高(P,<,0.05)。与模型组比较,生津润燥方各剂量组分别可升高CD8,+,T淋巴细胞水平,降低Th17淋巴细胞水平,升高小鼠Treg细胞水平,差异有统计学意义(P,<,0.05)。组织形态学观察显示,生津润燥方可抑制模型组小鼠颌下腺间质的淋巴细胞浸润。结论：生津润燥方通过调节干燥综合征小鼠淋巴细胞的应答作用,从而能改善SS小鼠的症状。

Abstract

Objective:To detect the immune induced Sjgren’s syndrome model rat’s CD4,+, CD25,+, Foxp3T cells (Treg) and Th17 cell level from peripheral blood,at the same time observe the improvement of the affected glands and the changes of peripheral blood lymphocyte subsets. MethodsMale BABL/C mice were divided into blank group, model group, hydroxychloroquine group and “Shengjin Renzao Prescription” high, middle, low-dose groups. The mice in all the groups except the blank group were established the sjogren’s syndrome in mice model by the immune inducing. Each group was intervened respectively. The level of CD4,+,、CD8,+,、CD4,+, CD25,+, Foxp3T cells and Th17 cell in the peripheral blood of mice was detected by using flow cytometry,and saliva,tear secretion of model rats was determined; At the end of its treatment,observe the change of submaxillary gland tissue morphology. Results:Compared with blank group, saliva and tear secretion of the mice from the first week to the fourth week in the model group decreased obviously(P,<,0.05)；compared with model group, saliva and tear secretion of the mice in “Shengjin Renzao Prescription” each dose group increased from the second, the third and the fourth week(P,<,0.05). Compared with blank group, the level of Th17 lymphocyte cell in model group increased(P,<,0.05). Compared with model group, “Shengjin Renzao Prescription” each dose group can increase level of CD8,+,T lymphocyte cell and decrease Th17 lymphocyte cell, and increase level of Treg cell, and there was significant difference(P,<,0.05). The morphologic observation showed that “Shengjin Renzao Prescription” can inhibit lymphocyte infiltration of submandibular gland stroma in model mice. Conclusion:“Shengjin Renzao Prescription” can improve the symptom of the SS in mice by adjusting the response function of lymphocytes.

关键词

干燥综合征CD4+CD8Th17CD25Foxp3T细胞

Keywords

Sjgren’s syndromeCD4+CD8Th17CD25Foxp3T cell

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The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4+ CD25+ Foxp3T/Th17 cells

DOI：

摘要

Abstract

关键词

Keywords

references

The research of “Shengjin Renzao Prescription” in the treatment of Sjgren’s syndrome and regulation mechanism of CD4⁺ CD25⁺ Foxp3T/Th17 cells