Effect of tonify kidney and drain dampness method on expression of IL-6, TGF-β in gouty arthritis rats

JIANG De-you; LIU Tong-tong; CHANG Jia-yi; XIE Ying; CHEN Fei; HAN Hai-wei; ZHANG Xu-dong; HAN Jie-ru

您当前的位置：

首页 >

文章列表页 >

Effect of tonify kidney and drain dampness method on expression of IL-6, TGF-β in gouty arthritis rats

更新时间：2022-08-31

- Effect of tonify kidney and drain dampness method on expression of IL-6, TGF-β in gouty arthritis rats
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 9, Pages: 77-80(2015)
- 作者机构：
  
  1. 黑龙江中医药大学,黑龙江,哈尔滨,150040
- 作者简介：
- 基金信息：
- DOI：
  CLC：
扫描看全文
JIANG De-you, LIU Tong-tong, CHANG Jia-yi, et al. Effect of tonify kidney and drain dampness method on expression of IL-6, TGF-β in gouty arthritis rats. [J]. Shanghai Journal of Traditional Chinese Medicine 49(9):77-80(2015)
DOI：

JIANG De-you, LIU Tong-tong, CHANG Jia-yi, et al. Effect of tonify kidney and drain dampness method on expression of IL-6, TGF-β in gouty arthritis rats. [J]. Shanghai Journal of Traditional Chinese Medicine 49(9):77-80(2015) DOI：

摘要

目的：建立痛风性关节炎(GA)大鼠模型,通过观察炎症因子表达的变化情况,探讨补肾利湿法防治痛风性关节炎的作用机制,为补肾利湿法防治痛风性关节炎提供依据方法：将84只SD大鼠随机分为空白对照组、模型组、中药对照组、西药对照组及中药低中高剂量组,每组12只,各组给予相应干预。除空白组外,其余大鼠于末次给药1 h后采用尿酸钠联合氧嗪酸钾诱导大鼠急性痛风性关节炎模型。采用HE染色,观察滑膜组织病理变化。应用ELISA法分别检测各组大鼠血清中白介素6(IL-6)及转化生长因子β(TGF-β)的含量结果：与空白对照组比较,造模各组血清中IL-6与TGF-β的水平均显著升高(P<0.05)。补肾利湿法处方中剂量组、高剂量组与模型组比较,血清中IL-6的浓度明显下降(P<0.05),TGF-β的浓度有所增加(P<0.05),与秋水仙碱组差异无统计学意义(P>0.05)。与模型组比较,痛风舒组和补肾利湿法处方低剂量组血清中IL-6的浓度差异有统计学意义(P<0.05),但TGF-β浓度差异无统计学意义(P>0.05) 结论：补肾利湿法可抑制急性痛风性关节炎大鼠IL-6的表达,促进TGF-β的表达,可防治痛风性关节炎。

Abstract

Objective:To explore the mechanism of tonify kidney and drain dampness method in the treatment of gouty arthritis, therefore, to provide the theory basis for the prevention and treatment of tonify kidney and drain dampness method of gouty arthritis Methods:Eighty four rats were randomly divided into seven groups:blank control group,model group,herbal control group,western medicine control group,high dosage herbal group,medium dosage herbal group and low dosage herbal group,twelve rats in each group.The uric acid sodium and oxygen polybenzoxazines potassium were used to induce rat acute gouty arthritis model. HE staining method was used to observe the pathological changes of synovial tissue. ELISA was used to detect the concentration of serum IL-6 and TGF-β in rats and analyze the data Results:Compared with the blank control group, levels of IL-6 and TGF-β in serum in each model groups significantly increased (P<0.05). Compared with model group, the concentration of serum IL-6 in high dosage herbal group and medium dosage herbal group decreased significantly(P<0.05), while the concentration of serum TGF-β increased (P<0.05), with no difference in colchicine group (P>0. 05). Compared with the model group, the concentration of serum IL-6 in herbal control group and low dosage herbal group decreased (P<0.05), but there were still differences compared with colchicine group (P<0.05). However, colchicine group and low dosage herbal group TGF-β concentration compared with the model group, no significant difference (P>0. 05) Conclusion:Tonify kidney and drain dampness method can inhibit the expression of IL-6 in rat acute gouty arthritis, promote the expression of TGF-β, so it has an effect on the prevention and treatment of gouty arthritis.

关键词

补肾利湿法痛风性关节炎白介素6转化生长因子β

Keywords

tonify kidney and drain dampness methodgouty arthritisIL-6TGF-β

references

Views

296

下载量

CSCD

CNKI被引量

Alert me when the article has been cited

提交

Tools

Publicity Resources

Fan Guanjie’s experience in treatment of acute gouty arthritis based on the theory of “eight methods of moving⁃definite sequence”

Regulatory effect of tonifying kidney and disinhibiting dampness therapy on fibroblast⁃like synoviocytes of gouty arthritis rats through NALP6 signal transduction pathway

Study on regulatory effect of Chinese herbal compound for invigorating kidney and removing dampness on NALP6 signaling pathway in rats with gouty arthritis

Clinical observation of the effects of Sanhuang Capsule combined with Jinhuang Ointment on acute gouty arthritis with syndrome of dampness-heat blockage

Experimental study of effects of Qinma Formula on airway remodeling in rats with chronic obstructive pulmonary disease

Related Author

No data

Related Institution

Department of Endocrinology， Zhaotong Hospital of Traditional Chinese Medicine

Department of Endocrinology， Guangdong Hospital of Traditional Chinese Medicine

Basic Medicine College， Heilongjiang University of Chinese Medicine

College of Basic Medicine, Heilongjiang University of Chinese Medicine

Department of Rheumatology,Suzhou Municipal Hospital of Traditional Chinese Medicine

⁰