Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β<sub>2</sub>-GP<sub>1</sub> and the expression of TLR4/NF-κB signaling pathway

FENG Xiao-ling; CHEN Lu; LI Na; ZHAO Yan; MA Li-ping; WANG Ling

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Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β₂-GP₁ and the expression of TLR4/NF-κB signaling pathway

更新时间：2022-08-31

- Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β₂-GP₁ and the expression of TLR4/NF-κB signaling pathway
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 8, Pages: 62-65(2015)
- 作者机构：
  
  1. 黑龙江中医药大学附属第一医院妇科,黑龙江,哈尔滨,150040
  2. 黑龙江中医药大学临床医学院,黑龙江,哈尔滨,150040
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FENG Xiao-ling, CHEN Lu, LI Na, et al. Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β₂-GP₁ and the expression of TLR4/NF-κB signaling pathway. [J]. Shanghai Journal of Traditional Chinese Medicine 49(8):62-65(2015)
DOI：

FENG Xiao-ling, CHEN Lu, LI Na, et al. Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β₂-GP₁ and the expression of TLR4/NF-κB signaling pathway. [J]. Shanghai Journal of Traditional Chinese Medicine 49(8):62-65(2015) DOI：

摘要

目的：研究隐丹参酮对抗心磷脂抗体(ACA)、抗β,2,糖蛋白1抗体(anti-β,2,-GP,1,)阳性模型SD大鼠抗体水平及TLR4/NF-κB 信号通路的影响方法：将SD雌性大鼠随机分为6组,即隐丹参酮组(0.03 μg/g)、丹参注射液组(6 μl/g)、阿司匹林组(0.04 μg/g)、水溶剂组(与隐丹参酮组相同体积吐温80与0.9%NaCl溶液的混合液)、模型组(与隐丹参酮组相同体积0.9%NaCl溶液)、空白组(与隐丹参酮组相同体积0.9%NaCl溶液)。采用腹腔注射地塞米松注射液及盐酸肾上腺素注射液造模,共16日。于造模完成后第1天开始灌胃给药,连续2周。造模完成后及灌胃结束后采血,采酶联免疫吸附法(ELISA法)检测血清中ACA、Anti-β,2,-GP,1,水平,再将雌雄大鼠合笼,受孕的第12天将大鼠处死。用ELISA法检测血清中肿瘤坏死因子α(TNF-α)的水平并计算活胎数。取大鼠子宫蜕膜组织,采用免疫组化链霉菌抗生物素蛋白过氧化物酶连结法(SP)法测定Toll样受体4(TLR4) 蛋白的表达水平结果：①隐丹参酮可以有效降低血清中ACA、Anti-β,2,-GP,1,的水平, 提高大鼠活胎数。②隐丹参酮可以使大鼠子宫蜕膜细胞内TLR4的表达水平降低, 从而降低血清中TNF-α水平,降低妊娠风险结论：隐丹参酮可有效降低ACA、Anti-β,2,-GP,1,阳性大鼠的流产率,其机制可能与抑制 TLR4/NF-κB 信号通路有关。

Abstract

Objective:To study the effects of cryptotanshinone on the antibody level of rats with the positive Anticardiolipin antibody and antiβ,2,-glycoprotein and the expression of TLR4/NF-κB Signaling Pathway Methods:The female SD rats were divided randomly into cryptotanshinone(0.03 μg/g)group, Salvia miltiorrhiza injection(6 μl/g)group, aspirin(0.04 μg/g)group, hydrosolvent(tween-80 saline at the same liquid volume) group, model group(normal saline at the same liquid volume) and blank group(NS at the same liquid volume). Fed the rats by gavage with medicine continuously from the first day for 2 weeks. ACA and Anti-β,2,-GP,1, levels were tested with the method of ELISA after the model was set up and fed by gavage, and then put the male and female rats into the same cage. The rats were sacrificed on the 12th day after pregnancy, serum level of TNF-α and the number of live births were detected by the method of ELISA. Decidual tissue were selected, and the expression of the TLR4 protein were tested by the method of SP Results:① Cryptotanshinone may effectively reduce the concentrations of ACA, Anti-β,2,-GP,1, and can increase the rate of live births effectively. ②The protein expression of TLR4 in decidual cells can be reduced by cryptotanshinone which resulting in lower TNF-α concentration to decrease the risk of miscarriage Conclusion:Cryptotanshinone has a protective effect on low pregnancy of rats with the positive of Anticardiolipin antibody and anti-β,2,-GP,1, which is partially via attenuating inflammatory through TLR4 / NF-κB signaling pathway.

关键词

隐丹参酮抗心磷脂抗体抗β2糖蛋白1抗体TLR4/NF-κB信号通路复发性流产

Keywords

cryptotanshinoneanticardiolipin antibodyanti-β2-glycoprotein 1TLR4/NF-κB signaling pathwayrecurrent spontaneous abortion

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Changhai Hospital Affiliated to the Second Military Medical University

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Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β2-GP1 and the expression of TLR4/NF-κB signaling pathway

DOI：

摘要

Abstract

关键词

Keywords

references

Effects of Cryptotanshinone on the antibody level of SD rats with the positive of Anticardiolipin antibody and anti-β₂-GP₁ and the expression of TLR4/NF-κB signaling pathway