Objective:To study the impact of “Jieyu Yubao Prescription” on estrous cycle, β-EP and pinopodes in chronic stress rats and discuss the regulatory mechanism of “Jieyu Yubao Prescription” on endometrial receptivity in chronic stress rats  Methods:80 SPF SD rats were randomly divided into 5 groups:normal control group, model group, “Jieyu Yubao Prescription” low-dose group, “Jieyu Yubao Prescription” middle-dose group, “Jieyu Yubao Prescription” high-dose group. Except the normal group, the remaining four groups of rats were established drug chronic stress model. From the 15th day of stimulation, the rats in the “Jieyu Yubao Prescription” middle-dose group were given the solution of “Jieyu Yubao Prescription”, intragastric administration capacity was 11.2 μl/g；normal group and model group were fed with an equal volume of purified water (11.2 μl/g); low-dose group and high-dose group were given 1/2 and 2 times of middle-dose group once a day, the administration capacity was 5.6 μl/g and 22.4 μl/g. The estrous cycle of rats was observed from the fourteenth day after model-building. All the rats in the five groups were made to copulate (female∶male=2∶1) 16∶00 on the 28th day of stimulation. On the 4th day after confirmation of pregnancy by Vaginal smears, the rats were killed at 16∶00 at the day of drug withdrawal. Serum β-EP was tested and endometrial VEGF levels was observed by Transmission Electron Microscope. Pregnancy rate and the average of implantation number were calculated  Results:① The general situation and estrous cycle:hair of the rats in the normal group, middle-dose group and high-dose group were clean and neat, active and with bright eyes and normal diet, body weights increased rapidly. Compared with model group, the time of estrous cycle of the rats increased obviously (P<0.05), and there was no significant difference compared with normal group(P>0.05).② The serum β-EP content:Compared with normal group, the serum β-EP of model group and low-dose group was significantly increased (P<0.05); compared with model group, the serum β-EP of middle-dose group and high-dose group was significantly decreased (P<0.05); the serum β-EP of middle-dose group and high-dose group was obviously lower that that in the low-dose group (P<0.05). ③ The expression of endometrial VEGF levels:compared with model group, the score of model group and low-dose group decreased obviously(P<0.05); there was no significant difference in score of high-dose group compared with normal group(P>0.05); the score of middle-dose group and high-dose group obviously higher than the normal group(P<0.05); the score of middle-dose group and high-dose group obviously higher than the low-dose group(P<0.05). ④ Compared with normal group, the average of implantation number of rats in model group and low-dose group decreased significantly (P<0.05), while there was no significant difference in middle-dose group and high-dose group (P>0.05). Compared with normal group, the average of implantation number of rats in middle-dose group and high-dose group increased significantly (P<0.05). The average of implantation number of rats in middle-dose group and high-dose group was higher than that of low-dose group (P<0.05); there was no significant difference in middle-dose group and high-dose group (P>0.05)  Conclusion:① “Jieyu Yubao Prescription” can improve or reduce chronic stress serum β-EP valuation effectively, and can reduce stress damage to the body, increase the body’s anti-stress ability, and shows a dose-dependent manner. ② “Jieyu Yubao Prescription” high-dose can significantly increase chronic stress in rats VEGF expression in the endometrium. ③ “Jieyu Yubao Prescription” can increase the expression of VEGF by balancing distribution of β-EP of the body due to the tension caused by the stress, and improve endometrial pinopodes maturity of endometrial epithelial cells, thereby improving the endometrial receptivity.