Objective:To observe the effect on the content of monoamine neurotransmitter and ATP enzyme by needling “Top siguan” in cerebral ischemia reperfusion rabbit,and to discuss the mechanism of nerve cells protection  Methods:rabbits were randomly divided into three groups :sham-operated group, model group and acupuncture group,and each group was divided into post-ischemia 1d,3d and 7d different periods subgroups. The focal cerebral ischemia and reperfusion model were established by the method of the middle cerebral artery occlusion(MCAO). They were performed by ischemia 2 h and then used for reperfusion. After 6 h,acupuncture group was accepted treatment by electroacupuncture “top Siguan”,once a day. The rabbits in the three groups were respectively executed and got cerebral tissue in 1 d,3 d and 7 d. Then they were determined the level of dopamine (DA),noradrenaline (NE) and 5-hydroxytryptamin (5-HT) by using fluorospectrophotometry,and detected the content of Na,+,-K,+,-ATP and Ca,2+,-ATP by using colorimetry  Results: Compared by sham-operated group in the same time,DA,NE and 5-HT were decreased obviously in model group especially in the 1 d group,but DA,NE and 5-HT somewhat increased with cerebral ischemia reperfusion time gone by. Na,+,-K,+,-ATP and Ca,2+,-ATP decreased obviously, the differences was statically significant (P,<,0.01). Compared with the model group in every time, the level of DA,NE and 5-HT, the content of Na,+,-K,+,-ATP and Ca,2+,-ATP were increased in different degree(P,<,0.01)  Conclusion:Acupuncture “Top siguan”can improve ATP enzyme metabolism,can reduce monoamine neurotransmitter release and inhibit monoamine neurotransmitter toxicity effect in cerebral ischemia reperfusion rabbit. so needling “Top siguan”can play a role of resistance ischemic injury.