Determination of lobetyolin in Platycodon grandiflorum from different parts and different areas by HPLC method

LIU Ying; FU Cheng-lin; LI Yue-ru; GUO Jing; WANG Ying-ping; LI Wei

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Determination of lobetyolin in Platycodon grandiflorum from different parts and different areas by HPLC method

更新时间：2022-08-31

- Determination of lobetyolin in Platycodon grandiflorum from different parts and different areas by HPLC method
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 7, Pages: 87-89(2015)
- 作者机构：
  
  1. 吉林农业大学中药材学院,吉林,长春,130118
  2. 中国农业科学院特产研究所,吉林,长春,130112
- 作者简介：
- 基金信息：
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LIU Ying, FU Cheng-lin, LI Yue-ru, et al. Determination of lobetyolin in Platycodon grandiflorum from different parts and different areas by HPLC method. [J]. Shanghai Journal of Traditional Chinese Medicine 49(7):87-89(2015)
DOI：

LIU Ying, FU Cheng-lin, LI Yue-ru, et al. Determination of lobetyolin in Platycodon grandiflorum from different parts and different areas by HPLC method. [J]. Shanghai Journal of Traditional Chinese Medicine 49(7):87-89(2015) DOI：

摘要

目的：采用HPLC法测定不同部位、不同产地桔梗中党参炔苷含量,为补充桔梗质量评价标准提供参考方法：色谱柱为Introject C,18, (250 mm×4.6 mm, 5 μm),以乙腈和水为流动相进行梯度洗脱,流速为1.0 ml/min,检测波长267 nm,柱温30 ℃ 结果：党参炔苷在0.065 625～1.05 g/L(R,2,=0.999 1)范围内与峰面积呈良好线性关系,精密度良好,平均回收率为98.41%。不同部位、不同产地桔梗药材中党参炔苷的含量范围为0.031 5~0.489 9 mg/g 结论：不同部位、不同产地的桔梗药材中党参炔苷的含量差异较大；本法准确可行、重复性好,可以用于桔梗及其制品中党参炔苷的含量检测。

Abstract

Objective:To supplement a reference for evaluation of quality standard of Platycodon grandiflorum through determining the contents of lobetyolin of Platycodon grandiflorum from different parts and different areas by HPLC Methods:Introject C,18, (250 mm×4.6 mm, 5μm) column was applied. The mobile phase was consisted of acetonitrile-water with gradient elution and flow rate of 1.0 mL·min-1. The detection wavelength was set at 267 nm and the column temperature was set at 30 ℃ Results:The calibration curve was linear in the range of 0.065625~1.05 g/L (R,2,=0.999 1),and the average recovery rate was 98.24%. The contents of lobetyolin in P. grandiflorum from different parts and different areas were varied between 0.0315 and 0.4899 mg·g-1 Conclusion:There was remarkable difference in contents of lobetyolin of Platycodon grandiflorum from different parts and different areas. The method in this paper is accurate,feasible and reproducible,which can be applied as the determination of lobetyolin in P. grandiflorum and its products.

关键词

桔梗党参炔苷HPLC质量标准

Keywords

Platycodon grandiflorumlobetyolinHPLCquality standard

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Laboratory of Traditional Chinese Medicine Processing, Shandong Academy of Chinese Medicine

Aquatic products 161 class, Yantai Research Institute of China Agricultural University

Institute of Chinese Materia Medica， Shanghai University of Traditional Chinese Medicine， The MOE Key Laboratory for Standardization of Chinese Medicines， The SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines， Shanghai R&D Center for Standardization of Chinese Medicines

Department of Pharmaceutical， Gongli Hospital， Shanghai Pudong New Area

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