Effects of Icariin on expressions of BMP-2 mRNA and Runx-2 mRNA in preosteoblastic cell lines OCT1

DA Wei-wei; ZHAO Yong-jian; WANG Yong-jun; CHENG Shao-dan; SHI Qi; TANG De-zhi

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Effects of Icariin on expressions of BMP-2 mRNA and Runx-2 mRNA in preosteoblastic cell lines OCT1

更新时间：2022-08-31

- Effects of Icariin on expressions of BMP-2 mRNA and Runx-2 mRNA in preosteoblastic cell lines OCT1
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 5, Pages: 90-94(2015)
- 作者机构：
  
  1. 上海中医药大学附属龙华医院,上海,200032
  2. 上海中医药大学脊柱病研究所,上海,200032
  3. 上海市光华中西医结合医院康复科,上海,200052
- 作者简介：
- 基金信息：
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DA Wei-wei, ZHAO Yong-jian, WANG Yong-jun, et al. Effects of Icariin on expressions of BMP-2 mRNA and Runx-2 mRNA in preosteoblastic cell lines OCT1. [J]. Shanghai Journal of Traditional Chinese Medicine 49(5):90-94(2015)
DOI：

DA Wei-wei, ZHAO Yong-jian, WANG Yong-jun, et al. Effects of Icariin on expressions of BMP-2 mRNA and Runx-2 mRNA in preosteoblastic cell lines OCT1. [J]. Shanghai Journal of Traditional Chinese Medicine 49(5):90-94(2015) DOI：

摘要

目的：探讨淫羊藿苷对成骨细胞增殖和分化的影响及作用机制方法：常规培养前期实验建立的前成骨细胞株OCT-1 细胞(OCT1 细胞),采用淫羊藿苷进行干预48 h,浓度分别为10 mg/L、30 mg/L及60 mg/L,并以BMP2 纯化蛋白(50 μg/L)为阳性对照。利用MTT法检测成骨细胞增殖率,实时荧光定量RT-PCR 检测成骨细胞中BMP2 mRNA、Runx2 mRNA 的表达结果：与正常对照组比较,淫羊藿苷能明显促进成骨细胞的增殖(P<0.05)；淫羊藿苷可显著提高成骨细胞中BMP2 mRNA、Runx2 mRNA 表达量(P<0.05) 结论：淫羊藿苷通过激活前成骨细胞中BMP信号通路上调Runx2的表达,从而诱导成骨细胞的增殖和分化。

Abstract

Objective:To observe the effect and mechanism of Icariin on proliferation and differentiation of osteoblasts Methods:The preosteoblastic cell lines OCT1 cells were cultured under the routine method, then intervened for 48 hours with Icariin, at the concentration of 10ug/mL, 30ug/mL and 60ug/mL respectively, and the purified protein BMP2 (50μg/L) as a positive control. MTT method was performed to detect the proliferation rate of osteoblasts, and RT-PCR analysis was performed to observe the expression of BMP2 mRNA, Runx2 mRNA Results:Compared with the normal control group, Icariin promoted the osteoblast proliferation obviously (P<0.05); Icariin increased the expression of BMP2 mRNA and Runx2 mRNA in osteoblasts significantly (P<0.05) Conclusion:Icariin can induce the proliferation and differentiation of osteoblasts by activating the BMP signaling pathway and up-regulating the expression of Runx2 in preosteoblastic cell lines.

关键词

细胞株成骨细胞淫羊藿苷细胞增殖实时荧光定量PCR

Keywords

cell lineosteoblasticariincell proliferationreal time fluorescent quantitative (PCR)

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