Effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402

JIANG Yan-xia; JI Peng-yan; WANG Ying; ZHU Wen-he; LUO Jun; XU Jun-jie; LU Xiao-jing

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Effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402

更新时间：2022-08-31

- Effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 3, Pages: 85-87(2015)
- 作者机构：
  
  1. 吉林医药学院生物化学教研室,吉林,吉林,132013
  2. 吉林医药学院生物化学实验室,吉林,吉林,132013
  3. 吉林医药学院直属医院,吉林,吉林,132013
- 作者简介：
- 基金信息：
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JIANG Yan-xia, JI Peng-yan, WANG Ying, et al. Effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402. [J]. Shanghai Journal of Traditional Chinese Medicine 49(3):85-87(2015)
DOI：

JIANG Yan-xia, JI Peng-yan, WANG Ying, et al. Effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402. [J]. Shanghai Journal of Traditional Chinese Medicine 49(3):85-87(2015) DOI：

摘要

目的：探讨海带多糖对人肝癌bel-7402细胞存活和凋亡的影响。方法： ①体外培养人肝癌bel-7402细胞,加入海带多糖(浓度分别为5、10、20、30 g/L)继续培养48 h,另将不用药物干预的肿瘤细胞组设为对照组；倒置显微镜观察细胞形态的变化,MTT 法测定细胞存活率。②体外培养人肝癌bel-7402细胞,设对照组及海带多糖组,海带多糖用药组分别加入海带多糖(10、20、30 g/L),采取相应干预措施。用流式细胞仪检测细胞周期和细胞凋亡情况。结果：①海带多糖(5、10、20、30 g/L)分别作用bel-7402细胞48 h后,随着海带多糖浓度的增加,bel-7402细胞逐渐变小、固缩、变形；细胞存活率明显降低。②海带多糖作用48 h后,bel-7402细胞凋亡率为(14.8 ±1.7) %,坏死率为(19.8±2.3)%,与对照组比较,差异有统计学意义(P＜0.05)。结论：海带多糖可抑制bel-7402细胞存活,促进bel-7402细胞凋亡及坏死。

Abstract

Objective:To observe the effect of laminarin on survival and apoptosis of human hepatocarcinoma cell of Bel-7402.Methods① Cultured human hepatocarcinoma cell of Bel-7402 in vitro, after added with the 5,0, 20,0, 40 g/L laminarin, cultured for 48 h. Without adding laminarin as the control group, we observed the changes of cells by inverted microscope and detected the cell viability by MTT assay. ② Cultured humanhepatocarcinoma cell of Bel-7402 in vitro, three groups:The control group, laminarin group and laminarin+ medicine group were treated with 10,0 and 30 g/L respectively. The cell cycle and apoptosis rate were analyzed by flow cytometer. Results:① After bel-7402 cells were cultured using 5,0, 20,0, 40 g/L laminarin for 48 h, with the increasement of laminarin concentration, bel-7402 cells were shrinked and changes. The cell survival rate was significantly decreased. ② After 48h, the bel-7402 cells apoptosis rate was (14.8 ±1.7)% and death rate was (19.8±2.3), with significant difference from the control group (P＜0.05). Conclusion:Laminarin can inhibit survival rate, and promote the apoptosis and necrocytosis of bel-7402 cells.

关键词

海带多糖bel-7402细胞细胞凋亡细胞周期

Keywords

laminarinbel-7402 cellapoptosiscell cycle

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