Objective:To discuss the effect of ginseng and scutellaria bionic extract (SQBE) on HIF-1α expression in HaCaT cells irradiated by UVB and to expound the possible mechanism of anti-UVB radiation on skin cancer. MethodsLogarithmic growth phase HaCaT cells were seeded in petri dishes containing 10% fetal bovine serum in DMEM medium, and cultured in 37 ℃, 5% CO,2, incubator; We divided into a control group, SQBE 1 group (12.5 μg·ml-1 SQBE), SQBE 2 group (25.0 μg·ml-1 SQBE), and SQBE 3 group (50.0 μg·ml-1 SQBE), each group was irradiated by UVB (0,0, 40,0 mJ·cm-2) 4 hours after administration. After 24 hours, the cell survival rate was detected by MTT method; expression of HIF-1αmRNA and HIF-1α protein were detected by real-time PCR and Western blotting.  Results:Compared with the control group at the same dose, the cell survival rate in the SQBE 3 group was increased statistically (P,<,0.05), and no significant difference was found between SQBE 1 group and SQBE 2 group (P,>,0.05); the expressions of HIF-1αmRNA and HIF-1α protein were decreased markedly in the SQBE3 group.  Conclusion:SQBE can prevent the UVB-induced skin cancer by influcing the expression of HIF-1α in cells possibly.