Effects of cyclic mechanical stretch on IL-1β-induced MMPs mRNA expression of synovioblast in human knee joints

LIU Yi-jie; YANG Song-bin; CHU Li-xi; LEE Shin-da; HU Jun; HUANG Zheng

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Effects of cyclic mechanical stretch on IL-1β-induced MMPs mRNA expression of synovioblast in human knee joints

更新时间：2022-08-31

- Effects of cyclic mechanical stretch on IL-1β-induced MMPs mRNA expression of synovioblast in human knee joints
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 2, Pages: 65-67(2015)
- 作者机构：
  
  1. 上海中医药大学康复医学院,上海,201203
  2. 中国医药大学物理治疗系,台湾,台中,40402
  3. 上海市光华中西医结合医院关节内科,上海,200052
- 作者简介：
- 基金信息：
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LIU Yi-jie, YANG Song-bin, CHU Li-xi, et al. Effects of cyclic mechanical stretch on IL-1β-induced MMPs mRNA expression of synovioblast in human knee joints. [J]. Shanghai Journal of Traditional Chinese Medicine 49(2):65-67(2015)
DOI：

LIU Yi-jie, YANG Song-bin, CHU Li-xi, et al. Effects of cyclic mechanical stretch on IL-1β-induced MMPs mRNA expression of synovioblast in human knee joints. [J]. Shanghai Journal of Traditional Chinese Medicine 49(2):65-67(2015) DOI：

摘要

目的：观察周期性牵张应力对IL-1β刺激的人膝骨关节滑膜成纤维细胞基质金属蛋白酶(MMP1、MMP3及MMP13)mRNA表达的影响方法：取第四代的人膝骨关节滑膜成纤维细胞,以每孔4×10,5,接种于由Ⅰ型胶原蛋白包被超薄弹性硅胶膜表面的6孔BioFlex细胞培养板,分为空白组(常规培养)、对照组(加入终浓度为10 μg/L的IL-1β刺激)和牵张组(加入终浓度为10 μg/L的IL-1β刺激,同时采用Flexcell细胞牵张拉伸应力加载系统对细胞进行周期性牵张力刺激),均连续干预3天；各组于牵张组实验结束后收集细胞,抽提滑膜细胞RNA,采用RT-PCR法检测细胞MMP1、MMP3和MMP13 mRNA的表达情况结果：与空白组比较,对照组MMP1、MMP3及MMP13 mRNA表达量明显升高,差异有统计学意义(P,<,0.05)；与对照组比较,牵张组MMP1、MMP13 mRNA表达量明显下降(P,<,0.05),MMP3 mRNA水平下降幅度不明显(P,>,0.05) 结论：周期性牵张应力(幅度为0-12%,频率为1 Hz,加载时间为60 min/d)能抑制人膝骨关节滑膜成纤维细胞MMP1、MMP13 mRNA的表达,从而对关节滑膜炎引起的软骨损伤起到保护作用。

Abstract

Objective:To investigate the role of cyclic mechanical stretch on IL-1β-induced MMPs mRNA expression in synovioblasts of human knee joints Methods:The fourth-generation synovioblasts of human knee joints were seeded onto type Ⅰ collagen-coated BioFlex 6-well culture plate at density of 4×10,5,/well, the following three different treatment groups were devised:normal group (untreated with IL-1β or cycle stretch), control group (treated with 10ng/ml IL-1β only), stretch group (treated with 10ng/ml IL-1β and subjected to cyclic mechanical stretch at a magnitude of 0-12%, frequency of 1Hz, and time of 60 min per day). After being stretched for 3 days by Flexcell-5000 tension system, RNA was extracted and RT-PCR was performed to detect the MMP1/3/13 mRNA level Results:Compared with the blank group, expressions of MMP1/3/13 mRNA in the control group were significantly increased (P,<,0.05); compared with the control group, expressions of MMP1/13 mRNA in the stretch group were decreased markedly (P,<,0.05), while he descend range of MMP3 mRNA was not obviously (P,>,0.05) Conclusion:Cyclic mechanical stretch (at a magnitude of 0-12%, frequency of 1Hz, and time of 60 min per day) may inhibit the MMP1/13 mRNA expression in synovioblasts of human knee joints, which may have the potential to protect cartilage damage induced by synovial inflammation.

关键词

滑膜成纤维细胞细胞培养周期性牵张力基质金属蛋白酶

Keywords

synovioblastcell culturecyclic mechanical stretchmatrix metalloproteinase

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