Objective: To observe the effects of different doses of “Qingzhi Hugan Prescription” on the expressions of Src family kinase (Hck, Lyn, Fgr) and Src-suppressed C kinase substrate in liver tissues of rats with nonalcoholic fatty liver disease, and to investigate its mechanism on NAFLD  Methods: Fifty-eight SD rats of two months were classified into normal group, except the normal group, others were feed with high-fat (80.5% ordinary fodder, 2% cholesterol, 12% lard, 5% egg yolk powder, 0.5% sodium cholic acid) for 8 weeks and the models were established. The models were randomized into model group, “Qingzhi Hugan Prescription” groups (large, medium and small dose). “Qingzhi Hugan Prescription”groups were gavaged with decotions, and the model group ang normal group was gavaged with 0.9% NaCl solution. After the experiment, the expressions of Hck, Lyn, Fgr and SSeCKS of the liver tissues were detected by immunohistochemistry  Results: Compared with model group, whatever the large, medium and small dose of the Chinese medicine groups, especially the large dose of “Qingzhi Hugan Prescription” group, the positive expressions of Hck and Lyn were decreased markedly (P＜0.01); compared with the normal group, the expression of SSeCKS in the model group was decreased markedly; in the Chinese medicine groups, the SSeCKS were expressed between the model group and normal group; It showed a trend of decrease along with the rise of the expressions of Lyn and Hck in the rat liver tissue with nonalcoholic fatty liver disease;Compared with model group, effect of “Qingzhi Hugan Prescription” on the expression of Fgr was insignificant (P>0.05)  Conclusion: Expressions of SSeCKS, Lyn and Hck were regulated by “Qingzhi Hugan Prescription”, which reduced inflammation of the liver. It can be used as one of the mechanisms for the treatment of NAFLD.