Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis

BAO Xiao-qing; WU Zhi-gang; YAN Peng-cheng; CHEN Song-lin; TAO Zheng-ming

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Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis

更新时间：2022-08-31

- Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 2, Pages: 86-89(2015)
- 作者机构：
  
  1. 温州医科大学药学院,浙江,温州,325005
  2. 浙江省亚热带作物研究所,浙江,温州,325005
- 作者简介：
- 基金信息：
- DOI：
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BAO Xiao-qing, WU Zhi-gang, YAN Peng-cheng, et al. Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis. [J]. Shanghai Journal of Traditional Chinese Medicine 49(2):86-89(2015)
DOI：

BAO Xiao-qing, WU Zhi-gang, YAN Peng-cheng, et al. Separation and determination of stilbenes ingredients in Dendrobii Officialis Caulis. [J]. Shanghai Journal of Traditional Chinese Medicine 49(2):86-89(2015) DOI：

摘要

目的：分离制备铁皮石斛质量指标性成分,并建立其含量的高效液相测定方法方法：硅胶、Sephadex LH-20等色谱学方法分离纯化铁皮石斛,波谱学特征鉴定化合物的结构；高效液相色谱法测定3,4-二羟基-5,4’-二甲氧基联苄 (DDB-1) 的含量, Phenomenex C,18,色谱柱 (250 mm×4.60 mm,5 μm),以乙腈-水溶液为流动相进行梯度洗脱,检测波长为230 nm 结果：共分离得到四个芪类化合物,分别为:3,4-二羟基-5,4’-二甲氧基联苄；3,4’-二羟基-5-甲氧基联苄；5-羟基-3,4’-二甲氧基联苄；4’-羟基-3’,5’-二甲氧基联苄。从中选取3,4-二羟基-5,4’-二甲氧基联苄 (DDB-1) 作为指标性成分,其在0.044～0.44 μg范围内呈现良好的线性关系(R,2, = 0.999 9)；不同种植基地的DDB-1含量差异较大,不同采收月份(3月份到5月份)的铁皮石斛中DDB-1含量差异不太明显结论：本研究建立的含量测定方法准确、可靠、简便,可用于铁皮石斛中DDB-1含量测定,为铁皮石斛质量评价提供了参考依据。

Abstract

Objective:To separate chemical indicator and establish a HPLC method for determining the indicator in herbs from Dendrobii Officialis Caulis Methods:The constituents were separated and purified by column chromatography on silica gel and Sephadex LH-20, as well as the chemical structures were determined by spectral data analyses. Determination of content of 3,4-dihydroxy-5,4’-dimethoxy bibenzyl (DDB-1) was performed by using high performance liquid chromatography. A Phenomenex-C,18, column (250 mm × 4.60 mm, 5 μm) was adopted with a mobile phase of acetonitrile-water, the runs were set at 230 nm Results:Four stilbenoids compounds were isolated and identified as 3,4-dihydroxy-5,4’-dimethoxybibenzyl, 3,4-dihydroxy-5-methoxybibenzyl, 5-hydroxy-3,4’-dimethoxybibenzyl, 4’-hydroxy-3’, 5’- dimethoxybibenzyl. DDB-1 was selected as an indicator to assess the quality of Dendrobii Officialis Caulis. This indicator showed a good linear relationship within the range of 0.044 ~ 0.44 μg (R,2,=0.999 9). The determination of DDB-1 in tested samples from different production base was most different, and it had little difference between each other if Dendrobii Officialis Caulis harvested in different month Conclusion:This proposed method was found to be accurate, reliable and simple, providing rapid and special determination of DDB-1, which could be used to control quality of Dendrobii Officialis Caulis.

关键词

铁皮石斛含量测定34-二羟基-54’-二甲氧基联苄高效液相色谱法

Keywords

Dendrobii Officialis Caulisdetermination34-dihydroxy-54’-dimethoxy bibenzylHPLC

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