Experimental study on vasoactive intestinal peptide mediated by electroacupuncture for the treatment of collagen-induced arthritis in rats

ZHU Jun; CHEN Xiao-yi; LI Lian-bo; ZHOU Yin; BING Xing-hong; CHEN Yun-fei

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Experimental study on vasoactive intestinal peptide mediated by electroacupuncture for the treatment of collagen-induced arthritis in rats

更新时间：2022-08-31

- Experimental study on vasoactive intestinal peptide mediated by electroacupuncture for the treatment of collagen-induced arthritis in rats
- Shanghai Journal of Traditional Chinese Medicine Vol. 49, Issue 1, (2015)
- 作者机构：
  
  1. 上海中医药大学附属岳阳中西医结合医院中心实验室,上海,200437
  2. 上海市针灸经络研究所,上海,200030
  3. 上海中医药大学附属岳阳中西医结合医院针灸科,上海,200437
- 作者简介：
- 基金信息：
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ZHU Jun, CHEN Xiao-yi, LI Lian-bo, et al. Experimental study on vasoactive intestinal peptide mediated by electroacupuncture for the treatment of collagen-induced arthritis in rats. [J]. Shanghai Journal of Traditional Chinese Medicine 49(1):null(2015)
DOI：

ZHU Jun, CHEN Xiao-yi, LI Lian-bo, et al. Experimental study on vasoactive intestinal peptide mediated by electroacupuncture for the treatment of collagen-induced arthritis in rats. [J]. Shanghai Journal of Traditional Chinese Medicine 49(1):null(2015) DOI：

摘要

目的：观察电针对胶原诱导性关节炎(CIA)大鼠的抗炎效应,探讨血管活性肠肽(VIP)介导电针抗炎的分子机制方法：将50只雄性SD大鼠随机分为5组,每组10只。除生理盐水组、电针生理盐水组外,CIA模型组、电针组和电针加VIP受体拮抗剂组用II型胶原诱导制备CIA模型。生理盐水组、CIA模型组不予干预,电针组、电针加VIP受体拮抗剂组和电针生理盐水组选择双侧“足三里”(ST 36)、“悬钟”(GB 39)予以电针干预(电针加VIP受体拮抗剂组在电针前腹腔注射VIP受体拮抗剂),每周3次,共计4周。电针干预期间,每周记录关节炎指数评分；实验结束后,HE染色观察大鼠滑膜组织的病理情况,免疫组化观察滑膜组织VIP的表达,ELISA法检测血清VIP、IFN-γ和IL-4的表达结果：实验结束时,电针组大鼠的关节炎指数评分低于CIA模型组和电针加VIP受体拮抗剂组。HE染色显示,电针组滑膜组织炎性细胞浸润、滑膜细胞增生等表现较CIA模型组显著减轻；免疫组化显示,电针组滑膜组织VIP的表达高于CIA模型组和电针加VIP受体拮抗剂组。电针组血清VIP、IL-4的表达高于CIA模型组和电针加VIP受体拮抗剂组(P<0.05,P<0.01),IFN-γ的表达低于CIA模型组和电针加VIP受体拮抗剂组(P<0.05) 结论：VIP介导了电针抗炎作用,其影响电针抗炎的机制可能和上调IL-4的表达水平,降低IFN-γ表达水平有关。

Abstract

Objective: To observe the anti-inflammatory effect of electroacupuncture on collagen-induced arthritis in rats and to explore the molecular mechanisms of vasoactive intestinal peptide (VIP) in mediating electroacupuncture Methods: Fifty male Sprague Dawley rats were randomly divided into five groups, with 10 rats in each group.Except for the normal saline (NS) group and electroacupuncture group, CIA rat models were established by collagen II.CIA models were induced by collagen type II.Normal saline group and model group were not intervened.Electroacupuncture group and electroacupuncture plus VIP receptor antagonist group, and electroacupuncture plus normal saline group were treated with electroacupuncture at Zusanli (ST-36) and Xuanzhong (GB-39).VIP receptor antagonist was injected before electroacupuncture three times a week, for 4 weeks.Scores of arthritis indexes were recorded during the electroacupuncture intervened period.The pathologic condition of synovial tissues was observed by HE.Expression of VIP was observed by immunohistochemical method, and expressions of serum VIP, IFN-γ and IL-4 were detected by ELISA Results: After intervention, the arthritis scores in electroacupuncture group were lower than that of the model group and electroacupuncture plus VIP receptor antagonist group.H Results: showed that there were inflammatory cells infiltration and fibroblasts proliferation in electroacupuncture group, and were lower than that of the model group.Immunohistochemisty showed that the VIP expression on synovial tissues in electroacupuncture group was significant higher than that of the model group, electroacupuncture plus VIP receptor antagonist group.Expression of VIP and IL-4 in electroacupuncture group were higher than that of the model group and electroacupuncture plus VIP receptor antagonist group (P<0.05, P<0.01); IFN-γ expression in the electroacupuncture group was lower than that of the model group and electroacupuncture plus VIP receptor antagonist group (P<0.05) Conclusion: Electro-acupuncture on Zusanli (ST-36) and Xuanzhong (GB-39) shows significant inhibition role of arthritis in CIA rats.Its mechanism may be associated with up-regulating the IL-4 expression and down-regulating the IFN-γ expression.

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关节炎胶原诱导大鼠电针血管活性肠肽

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