Objective: To investigate the effect “Tongjie Oral Solution” on IL-6 and TNF-α in serum and the ileum of rats with multiple organ dysfunction syndrome (MODS).Methods Fourty-eight male SD rats were randomly divided into 6 groups:control group, model group, positive control group, low dose treatment group, medium dose treatment group and high dose treatment group, with 8 rats in each group.Intragastric administration in advance, 2 days later, intravenous inject Lipopolysaccharide (LPS) of 8mg/kg to build rat models.The vital signs such as electrocardiogram, temperature, breathing extent and respiratory rate; serum indexes such as AST, ALT, CK and Cr; levels of IL-6 and TNF-αin the serum and ileum were detected.Results There were significant differences in the heart rate, temperature, respiratory rate, serum indexes (contents of AST, ALT, CK and Cr in the serum), and contents of TNF-α and IL-6 between the blank group and model group (P＜0.05, P＜0.01).There were the significant differences in the heart rate and temperature between the model group and positive control group (P<0.05); There were the significant difference in the heart rate among the low-, medium- and high-dose treatment groups (P<0.05, P<0.01). There were significant differences in the respiratory rate, serum indexes and IL-6 level in the ileum between the model group and positive control group (P<0.05, P<0.01); there were significant differences in the respiratory rate, serum indexes, TNF-α level and IL-6 level in the serum and ileum between the model group and high-dose treatment group (P<0.05, P<0.01); there were significant differences in the serum indexes, IL-6 in the serum and ileum between the model group and medium-dose treatment group (P＜0.05, P＜0.01); There was a significant difference in the Cr level between the model group and low-dose treatment group (P<0.01).There was a significant difference in the levels of ALT, AST, TNF-α and IL-6 in the serum and IL-6 in the ileum between the low and high-dose treatment groups, and there was a significant difference in the ALT level between the low and medium-dose treatment groups (P＜0.05, P＜0.01).There were significant differences in the contents of AST and ALT bwteen the positive control group and low-dose treatment group, and with significant differences in the IL-6 of the serum and ileum between the positive control group and high-dose treatment group (P＜0.05, P＜0.01).Conclusion “Tongjie Oral Solution” can effectively reduce the levels of IL-6 and TNF-α in both serum and ileum of rats with MODS reduced by lipopolysaccharide.The curative effect was markedly coincident with the dosage of the solution.